Method for producing vanillin
AssigneeFirmenich SA
InventorFredi Bruhlmann
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Abstract
The present disclosure relates to a novel method of producing vanillin and/or derivatives thereof by applying improved biocatalysts. Also provided herein are expression systems for preparing said improved biocatalysts. Moreover provided herewith are novel enzyme mutants, corresponding coding sequences and vectors applicable in the biochemical production of vanillin. The present disclosure further provides recombinant host cells or organisms genetically modified for improved functional expression of biocatalysts, as well as recombinant host cells or organisms useful to produce vanillin.
Claims17
An expression system for a recombinant expression of a polypeptide having isoeugenol oxidizing activity, which expression system comprises a single nucleic acid construct carrying:
- a. a nucleotide sequence (A) encoding a polypeptide having enzymatic isoeugenol oxidizing activity, and
- b. at least one nucleotide sequence (B) encoding at least one helper polypeptide which alone or in cooperation assist in a functional expression of the polypeptide, encoded by said nucleotide sequence (A);
- wherein said expression system provides for a co-expression of said nucleotide sequences (A) and (B), and
- wherein said nucleotide sequence (A) comprises an amino acid sequence having at least 85% sequence identity to SEQ ID NO:
- 4 and comprising at least one mutation in an amino acid sequence position selected from T52, Q74, D440, and optionally at least one further mutation in an amino acid sequence position selected from N120, T121, F281, M298, and L470.
A polypeptide having isoeugenol oxidizing activity that comprises an amino acid sequence having at least 95% sequence identity to SEQ ID NO:
- 4 and comprising at least one mutation in an amino acid sequence position selected from T52, Q74, D440 and optionally at least one further mutation in an amino acid sequence position selected from N120, T121, F281, M298, and L470.
Dependencies are inferred from the claim text.