Delivery across cell plasma membranes
AssigneeAvectas Limited
InventorsMichael Maguire, Shirley O'Dea
Life of this patent
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Abstract
Delivering a payload across a plasma membrane of a cell includes providing a population of cells and contacting the population of cells with a volume of an aqueous solution. The aqueous solution includes the payload and alcohol content greater than 5 percent concentration. The volume of the aqueous solution may be a function of exposed surface area of the population of cells, or may be a function of a number of cells in the population of cells. Related compositions, apparatus, systems, techniques, and articles are also described.
Claims73
A method for delivering a payload across a plasma membrane of a cell, comprising:
- providing a population of cells;
- and
- spraying the population of cells with a volume of aqueous solution, the aqueous solution including the payload and an alcohol at greater than 5 percent concentration, less than 46 mM salt, less than 121 mM sugar, and less than 19 mM buffering agent,
- wherein the salt is present in the aqueous solution and selected from a group consisting of NaCl, KCl, Na2HPO4, C2H3O2NH4 and KH2PO4, and
- wherein the volume is a function of:
- (i) exposed surface area of the population of cells;
- or (ii) a number of cells in the population of cells.
A composition for delivering a payload across a plasma membrane of a cell, the composition comprising an aqueous solution including the payload, an alcohol at greater than 5 percent concentration, less than 46 mM salt, less than 121 mM sugar, and less than 19 mM buffering agent, and wherein the salt is present in the aqueous solution and selected from a group consisting of NaCl, KCl, Na2HPO4, C2H3O2NH4 and KH2PO4.
A composition for delivering a payload across a plasma membrane of a cell, the composition comprising an aqueous solution including the payload, an alcohol at greater than 5 percent concentration, less than 46 mM salt, less than 121 mM sugar, and less than 19 mM buffering agent, and wherein the sugar is present in the aqueous solution and comprises sucrose.
A composition for delivering a payload across a plasma membrane of a cell, the composition comprising an aqueous solution including the payload, an alcohol at greater than 5 percent concentration, less than 46 mM salt, less than 121 mM sugar, and less than 19 mM buffering agent, and wherein the buffering agent is present in the aqueous solution and comprises 4-2-(hydroxyethyl)-1-piperazineethanesulfonic acid.
A composition for delivering a payload across a plasma membrane of a cell, the composition comprising an aqueous solution including the payload, an alcohol at greater than 5 percent concentration, less than 46 mM salt, less than 121 mM sugar, and less than 19 mM buffering agent, and wherein the aqueous solution comprises a hypotonic solution.
A composition for delivering a payload across a plasma membrane of a cell, the composition comprising an aqueous solution including the payload, an alcohol at greater than 5 percent concentration, less than 46 mM salt, less than 121 mM sugar, and less than 19 mM buffering agent, and wherein the aqueous solution comprises 12 mM KCl, 32 mM sucrose, and 25% v/v ethanol.
A composition for delivering a payload across a plasma membrane of a cell, the composition comprising an aqueous solution including the payload, an alcohol at greater than 5 percent concentration, less than 46 mM salt, less than 121 mM sugar, and less than 19 mM buffering agent, and wherein the aqueous solution further comprises 12 mM NH4.
Dependencies are inferred from the claim text.