Plants having enhanced tolerance to insect pests and related constructs and methods involving insect tolerance genes
AssigneeSinobioway Bio-Agriculture Group Co Ltd Pioneer Overseas Corporation
InventorsGuihua Lu, Guanfan Mao, Guokui Wang, Feng Zhong
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Abstract
The disclosure discloses isolated polynucleotides and polypeptides, and recombinant DNA constructs useful for conferring improved tolerance in plants to insect pests; compositions (such as plants or seeds) comprising these recombinant DNA constructs; and methods utilizing these recombinant DNA constructs. The recombinant DNA constructs comprise a polynucleotide operably linked to a promoter that is functional in a plant, wherein the polynucleotides encode insect tolerance polypeptides.
Claims9
A transgenic plant, plant cell or seed, comprising in its genome a recombinant DNA construct comprising a polynucleotide encoding a polypeptide comprising an amino acid sequence having at least 95% identity to SEQ ID NO:
- 6 operably linked to at least one heterologous regulatory element, wherein the plant, plant cell or seed has an increased tolerance to a Lepidopteran insect pest as compared to a control plant not comprising the recombinant DNA construct.
A method of increasing tolerance in a plant to a Lepidopteran insect pest, comprising
- (a) introducing into a regenerable plant cell a recombinant DNA construct comprising a polynucleotide operably linked to at least one regulatory element, wherein the polynucleotide encodes a polypeptide having an amino acid sequence of at least 95% identity compared to SEQ ID NO:
- 6;
- (b) regenerating a transgenic plant from the regenerable plant cell after step (a), wherein the transgenic plant comprises in its genome the recombinant DNA construct;
- and
- (c) obtaining a progeny plant derived from the transgenic plant of step (b), wherein the progeny plant comprises in its genome the recombinant DNA construct and exhibits increased tolerance to a Lepidopteran insect pest when compared to a control plant not comprising the recombinant DNA construct.
A method of evaluating tolerance in a plant to a Lepidopteran insect pest, comprising:
- (a) introducing into a regenerable plant cell a recombinant DNA construct comprising a polynucleotide operably linked to at least one regulatory element, wherein the polynucleotide encodes a polypeptide having an amino acid sequence of at least 95% identity when compared to SEQ ID NO:
- 6;
- (b) regenerating a transgenic plant from the regenerable plant cell after step (a), wherein the transgenic plant comprises in its genome the recombinant DNA construct;
- (c) obtaining a progeny plant derived from the transgenic plant, wherein the progeny plant comprises in its genome the recombinant DNA construct;
- and
- (d) evaluating the progeny plant for tolerance to a Lepidopteran insect pest compared to a control plant not comprising the recombinant DNA construct.
Dependencies are inferred from the claim text.