Back to results
US 11,447,787 B2Granted
Generation of haploid plants
AssigneeThe Regents of the University of California
InventorArundhati Maheshwari
Life of this patent
Estimating the term…
Abstract
Methods of making haploid plants.
Claims13
A method of targeting a kinetochore protein for degradation, the method comprising:
- a. introducing into a gamete cell of a parent plant a polypeptide comprising a peptide sequence linked to a protease cleavage site linked to N-degron linked to a kinetochore protein, wherein the peptide sequence is of sufficient length to block the polypeptide in an N-degron-dependent manner, and wherein the kinetochore protein is CENH3, and;
- b. introducing into the gamete cell of a parent plant a protease that targets the protease cleavage site, thereby releasing the peptide sequence such that the N-degron is at the amino terminus of the polypeptide, thereby targeting the polypeptide for degradation.
A plant comprising:
- a first expression cassette comprising a promoter operably linked to a polynucleotide encoding a polypeptide comprising a peptide sequence linked to a protease cleavage site linked to N-degron linked to a kinetochore protein, wherein the peptide sequence is of sufficient length to block the polypeptide in an N-degron-dependent manner and wherein the kinetochore protein is CENH3, and
- a second expression cassette comprising a heterologous promoter linked to a second polynucleotide encoding the protease, wherein the heterologous promoter is specific for a gamete lineage.
An expression cassette comprising a promoter operably linked to a polynucleotide encoding a polypeptide comprising a peptide sequence linked to a protease cleavage site linked to N-degron linked to a kinetochore protein, wherein the kinetochore protein is CENH3.
Dependencies are inferred from the claim text.