US 11,447,786 B2 · Systems and methods for cellular reprogramming of a plant cell

Abstract

Plant cell fate and development is altered by treating cells with cellular reprogramming factors. Embryogenesis inducing morphogenic developmental genes are used as cellular reprogramming factors, specifically comprising polypeptides or polynucleotides encoding gene products for generating doubled haploids or haploid plants from gametes. Maize microspores treated by contacting the isolated cells with an exogenous purified, recombinant embryogenesis inducing morphogenic developmental gene polypeptide results in embryogenesis. The gametes of a maize plant develop into embryoids when transformed with a genetic construct including regulatory elements and structural genes capable of acting in a cascading fashion to alter cellular fate of plant cells. Developmental morphogenic proteins expressed from a genetic construct are used for ex situ treatment methods and for in planta cellular reprogramming.

Claims5

Dependent claims

A method of generating a haploid plant embryo comprising:

(a) providing a plant microspore with an embryogenesis inducing compound to promote microspore embryogenesis in the plant microspore, wherein the embryogenesis inducing compound is hemin or a kinase inhibitor selected from N-[(2R)-2,3-dihydroxypropoxy]-3,4-difluoro-2-(2-fluoro-4-iodoanilino)benzamide, anthra(1,9-cd)pyrazol-6(2H)-one:4-(4-Fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)1H-imidazole, or N-benzyl-2-(pyrimidin-4-ylamino)-1,3-thiazole-4-carboxamide;
(b) isolating an embryogenic microspore from the plant microspore;
and
(c) culturing the embryogenic microspore to generate the haploid plant embryo.

1 more element

Dependencies are inferred from the claim text.

Facts

Application

16/755,432Filed 12 Oct 2018 · us provisional application

Priority

13 Oct 2017

Published

US 2020/0263189 A120 Aug 2020

Granted

20 Sep 2022

Term ends

Not estimatedThe source records don’t support an estimate.

Classification6 codes

C12N 15/823
Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation: reproductive tissue-specific promoters
A01H 1/08
Processes for producing mutations, e.g. treatment with chemicals or with radiation: methods for producing changes in chromosome number
C12N 9/226
Clustered regularly interspaced short palindromic repeats [CRISPR]-associated [CAS] enzymes: class 2 CAS enzyme complex, e.g. single CAS protein
C12N 2310/20
Type of nucleic acid: involving clustered regularly interspaced short palindromic repeats [CRISPR]

Systems and methods for cellular reprogramming of a plant cell

Life of this patent

Systems and methods for cellular reprogramming of a plant cell

Life of this patent

Abstract

Claims5