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US 11,447,783 B2Granted
Reduction in acetate production by yeast over-expressing PAB1
AssigneeDanisco US Inc.
InventorsMin Qi, Paula Johanna Maria Teunissen, Quinn Qun Zhu
Life of this patent
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Abstract
Described are compositions and methods relating to modified yeast that over-express polyadenylate-binding protein (PAB1). The yeast produces a deceased amount of acetate compared to parental cells. Such yeast is particularly useful for large-scale ethanol production from starch substrates where acetate in an undesirable end product.
Claims15
Modified yeast cells derived from parental yeast cells, the modified cells comprising:
- (a) a genetic alteration that causes the modified cells to produce an increased amount of PAB1 polypeptides compared to the parental cells, wherein the genetic alteration comprises an introduction into the parental cells of a nucleic acid capable of directing the expression of a PAB1 polypeptide to a level above that of the parental cell grown under equivalent conditions;
- and
- (b) one or more heterologous genes of a phosphoketolase pathway;
- and
- wherein the modified cells produce during fermentation a decreased amount of acetate compared to the amount of acetate produced by the parental cells under identical fermentation conditions.
2 more elements
A method for decreasing the production of acetate from yeast cells grown on a carbohydrate substrate, comprising:
- introducing into parental yeast cells a genetic alteration that increases the production of PAB1 polypeptides compared to the amount produced in the parental cells, wherein the genetic alteration comprises introducing into the parental cells a nucleic acid capable of directing the expression of a PAB1 polypeptide to a level above that of the parental cell grown under equivalent conditions.
Dependencies are inferred from the claim text.