Host cells for use in an inducible coexpression system
AssigneeAbSci LLC
InventorsSean McClain, Mark Valasek
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Abstract
The present invention provides host cells for use in an inducible coexpression system that is capable of controlled induction of expression of each gene product.
Claims30
A host cell suitable for expression of a gene product from at least one of two or more inducible promoters selected from the group consisting of Escherichia coli sugar-inducible promoters and propionate-inducible promoters, wherein at least one of said inducible promoters is responsive to an inducer that is different than the inducer of another of said inducible promoters, and wherein none of said inducible promoters is a lactose-inducible promoter;
- wherein the host cell has an altered gene function of a gene that affects the reduction/oxidation environment of the host cell cytoplasm;
- and
- wherein the host cell has a reduced level of gene function of a gene encoding a protein that metabolizes a first inducer of a first one of said inducible promoters;
- and
- wherein the host cell has a reduced level of gene function of a gene encoding a protein involved in biosynthesis of a second inducer of a second one of said inducible promoters, wherein the second inducer is different than the first inducer.
A host cell suitable for expression of a gene product from at least one of two or more inducible promoters selected from the group consisting of Escherichia coli sugar-inducible promoters and propionate-inducible promoters, wherein at least one of said inducible promoters is responsive to an inducer that is different than the inducer of another of said inducible promoters, and wherein none of said inducible promoters is a lactose-inducible promoter;
- wherein the host cell has an altered gene function of a gene that affects the reduction/oxidation environment of the host cell cytoplasm;
- and
- wherein the host cell has a reduced level of gene function of a gene encoding a protein that metabolizes a first inducer of a first one of said inducible promoters;
- and
- wherein the host cell has a reduced level of gene function of a gene encoding a protein that metabolizes a second inducer of a second one of said inducible promoters, wherein the second inducer is different than the said first inducer.
An E. coli host cell suitable for expression of a gene product from at least one of two or more inducible promoters, wherein the inducible promoters comprise an L-arabinose-inducible promoter and a propionate-inducible promoter, and
- wherein the host cell has an altered gene function of a gene that affects the reduction/oxidation environment of the host cell cytoplasm;
- and
- wherein the host cell has a genome that has been modified to have a deletion within the araBAD operon that results in a reduced level of gene function of a gene encoding a protein that metabolizes L-arabinose, and a deletion within the scpA-argK-scpBC operon that results in a reduced level of gene function of a gene encoding a protein involved in biosynthesis of propionate.
Dependencies are inferred from the claim text.