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AssigneesThe Broad Institute, Inc., President and Fellows of Harvard College
InventorsDavid R. Liu, Andrew Vito Anzalone, Gregory Newby, Kelcee Everette
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Compositions and methods are provided herein for conducting prime editing of a target DNA molecule (e.g., a genome) that enables the incorporation of a nucleotide change and/or targeted mutagenesis. The compositions include fusion proteins comprising nucleic acid programmable DNA binding proteins (napDNAbp) and a polymerase (e.g., reverse transcriptase), which is guided to a specific DNA sequence by a modified guide RNA, named a PEgRNA. The PEgRNA has been altered (relative to a standard guide RNA) to comprise an extended portion that provides a DNA synthesis template sequence which encodes a single strand DNA flap which is synthesized by the polymerase of the fusion protein and which becomes incorporated into the target DNA molecule.
A method for site-specific modification of a double-stranded target DNA sequence, the method comprising:
Dependencies are inferred from the claim text.