Bacillus lentus subtilisin protease variants and compositions comprising the same

An isolated subtilisin variant, wherein said subtilisin variant is a mature form having proteolytic activity and comprises an amino acid sequence comprising the combination of amino acid substitutions S188D-E271F, wherein the total net charge of the variant is 0, +1, +2, +3, +4, +5, −1, −2, −3, −4, or −5 relative to the total net charge of the Bacillus lentus subtilisin GG36 protease, and wherein the total net charge is obtained by one or more substitutions selected from:

• N43D, R45T, N62E, N76D, S101D, P129E, A158E, G159D, G159E, S166D, S188D, A230E, N18R, G20K, G20R, T22R, S24R, N43R, G118R, Q245R, H249R, N269R, E271F, and E271L, wherein the variant is a variant of a parent protease Bacillus lentus subtilisin GG36 protease comprising the amino acid sequence shown in SEQ ID NO:
• 2, and wherein amino acid positions of the protease variant are numbered according to the numbering of corresponding amino acid positions in the amino acid sequence of Bacillus amyloliquefaciens subtilisin BPN′ shown in SEQ ID NO:1, and wherein the variant has at least 90% amino acid sequence identity over its full length to SEQ ID NO:
• 2.