Genetically engineered Vibrio sp. and uses thereof
AssigneeCodex DNA, Inc.
InventorsMatthew T Weinstock, Christopher M. Wilson, Eric D. Hesek
Life of this patent
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Abstract
The present disclosure relates to the seminal discovery of a generation and use of genetically engineered Vibrio sp. Provided is the use of the genetically engineered bacteria for the construction, maintenance, manipulation, and/or propagation of DNA constructs; protein expression; protein secretion; vectors and other metabolic tools; metabolic engineering; expression of cellular extracts for cell-free biology; shuttle vectors; cloning vectors; and for synthetic biology applications. The disclosure also relates to the use of the replication machinery of Vibrio sp. as a cloning or expression vector for replication of recombinant DNA constructs. The disclosure also relates to methods of use of the above.
Claims18
A Vibrio natriegens organism comprising:
- a recombinant nucleic acid comprising a nucleotide sequence encoding a T7 RNA polymerase and a recombinant regulatory element 5′ to the nucleotide sequence encoding the T7 RNA polymerase that regulates expression of the T7 RNA polymerase;
- wherein the recombinant nucleic acid further encodes a modified ribosome binding site 5′ to the nucleotide sequence encoding the T7 RNA polymerase;
- and
- wherein the ribosome binding site comprises the nucleotide sequence of SEQ ID NO:
- 8, or the nucleotide sequence of SEQ ID NO:
- 8, except for 1, 2, or 3 nucleotide substitution modification(s) and wherein the ribosome binding site regulates translation of the T7 RNA polymerase.
Dependencies are inferred from the claim text.