In vitro methods of chemical conversion using non-stereospecific glutathione lyases

A method of chemical conversion, comprising contacting a first compound in vitro with a non-stereospecific glutathione lyase to yield a second compound, wherein:

• the non-stereospecific glutathione lyase comprises:
• an amino acid sequence at least 80% identical to any of:
• SEQ ID NO:
• 18 (NaGSTNu);
• residues 21-313 of SEQ ID NO:
• 20 (recombinant
• NaGSTNu);
• SEQ ID NO:
• 22 (SYK6GSTNu);
• residues 21-324 of SEQ ID NO:
• 24 (recombinant
• SYK6GSTNu);
• SEQ ID NO:
• 26 (ecYghU);
• residues 21-313 of SEQ ID NO:
• 28 (recombinant
• ecYghU);
• SEQ ID NO:
• 30 (ecYfcG);
• SEQ ID NO:
• 32 (ssYghU);
• and
• SEQ ID NO:
• 36 (PcUre2pB 1);
• and
• at least four of:
• threonine or a conservative variant of threonine at a position corresponding to position 51 of SEQ ID NO:18 (NaGSTNu);
• asparagine or a conservative variant of asparagine at a position corresponding to position 53 of SEQ ID NO:18 (NaGSTNu);
• glutamine or a conservative variant of glutamine at a position corresponding to position 86 of SEQ ID NO:18 (NaGSTNu);
• lysine, a conservative variant of lysine, arginine, or a conservative variant of arginine at a position corresponding to position 99 of SEQ ID NO:18 (NaGSTNu);
• isoleucine or a conservative variant of isoleucine at a position corresponding to position 100 of SEQ ID NO:18 (NaGSTNu);
• glutamate or a conservative variant of glutamate at a position corresponding to position 116 of SEQ ID NO:18 (NaGSTNu);
• serine, threonine, a conservative variant of serine, or a conservative variant of threonine at a position corresponding to position 117 of SEQ ID NO:18 (NaGSTNu);
• and
• arginine or a conservative variant of arginine at a position corresponding to position 177 of SEQ ID NO:18 (NaGSTNu);
• the first compound has a structure of Formula I or a salt thereof:
• wherein:
• R1, R2, and R3 are each independently —H, —OH, —O-alkyl, —O-lignin, or -lignin;
• R4 is —H, —OH, —SH, —COOH, —SO3H, or —O-lignin;
• and
• SG is glutathione bound in an S or R configuration;
• and
• the second compound has a structure of Formula II or a salt thereof:
• wherein:
• R1, R2, and R3 are each independently —H, —OH, —O-alkyl, —O-lignin, or -lignin;
• and
• R4 is —H, —OH, —SH, —COOH, —SO3H, or —O-lignin.