In vitro model for blood-brain barrier and method for producing in vitro model for blood-brain barrier

An in vitro model for blood-brain barrier, in which a sheet of conditionally immortalized astrocytes, a porous membrane, a sheet of conditionally immortalized brain pericytes, and a sheet of conditionally immortalized brain microvascular endothelial cells are laminated in this order from the bottom, or a sheet of conditionally immortalized microvascular endothelial cells, a sheet of conditionally immortalized brain pericytes, a porous membrane, and a sheet of conditionally immortalized astrocytes are laminated in this order from the bottom,

• wherein the conditionally immortalized brain microvascular endothelial cells, the conditionally immortalized brain pericytes, and the conditionally immortalized astrocytes are produced by introducing a temperature-sensitive SV40 large T antigen gene into primary cultured brain microvascular endothelial cells, primary cultured brain pericytes, and primary cultured astrocytes, respectively.

An in vitro model for blood-brain barrier, in which a sheet of conditionally immortalized astrocytes, a porous membrane, a sheet of conditionally immortalized brain pericytes, a laminin layer, and a sheet of conditionally immortalized brain microvascular endothelial cells are laminated in this order from the bottom, or a sheet of conditionally immortalized microvascular endothelial cells, a laminin layer, a sheet of conditionally immortalized brain pericytes, a porous membrane, and a sheet of conditionally immortalized astrocytes are laminated in this order from the bottom,

• wherein the conditionally immortalized brain microvascular endothelial cells, the conditionally immortalized brain pericytes, and the conditionally immortalized astrocytes are produced by introducing a temperature-sensitive SV40 large T antigen gene into primary cultured brain microvascular endothelial cells, primary cultured brain pericytes, and primary cultured astrocytes, respectively.