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US 11,447,750 B2Granted
System and method for extraction and cryopreservation of bone marrow
AssigneeOssium Health, Inc.
InventorsErik John Woods, Brian H. Johnstone, Dongsheng Gu, Aubrey Marie Sherry, Kelsey Gwen Musall, John R. Woods, James Hardin, Alan Hooks
Life of this patent
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Abstract
Methods are provided for extracting bone marrow cells from bone obtained from deceased donors, for preparing the bone marrow for cryopreservation and for obtaining desired cells from cryopreserved and fresh bone marrow.
Claims20
A method for recovering vertebral bone marrow mesenchymal stromal/stem cells (vBM-MSCs) and for recovering vertebral bone adherent mesenchymal stromal/stem cells (vBA-MSC) from cadaver bone or cadaver bone fragments, the method comprising steps of:
- (a) obtaining cadaver bone, cadaver bone fragments, ground cadaver bone, or preparing ground cadaver bone from cadaver bone or cadaver bone fragments;
- (b) contacting the ground cadaver bone with a grinding medium under conditions sufficient to separate bone marrow comprising vBM-MSCs from the ground cadaver bone;
- (c) capturing a byproduct ground cadaver bone on a filter or sieve and collecting a filtrate that passes thru the filter or sieve, thereby recovering the bone marrow comprising the vBM-MSCs;
- (d) incubating the byproduct captured ground cadaver bone in a digestive solution comprising collagenase and neutral protease, thereby liberating vBA-MSCs from the captured ground cadaver bone;
- and
- (e) collecting the liberated vBA-MSCs, thereby recovering the vBA-MSC;
- wherein steps (b) and (c) precede step (d);
- wherein at least 2×108 vBA-MSC are obtained from 100 g of captured byproduct ground cadaver bone.
5 more elements
Dependencies are inferred from the claim text.