Transparent disc-shaped microparticles coated with polydopamine
AssigneeUniversity-Industry Cooperation Group of Kyung Hee University
InventorsTong In Oh, Sung Hyun Kim, Wook Park, Eun Ah Lee
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Abstract
The present invention relates to transparent disc-shaped microparticles having polydopamine-modified surfaces and cell culture using the same. When the disc-shaped microparticles coated with polydopamine according to the present invention are used in cell culture, cell adhesive properties are improved and an inverted microscope may be employed to observe cells, which are major advantages when compared to conventional spherical microcarriers.
Claims4
A disc-shaped microparticle for cell culture, comprising a polydopamine-coated surface layer, wherein the disc-shaped microparticle is formed by photocuring polymerization of a photocurable substance having an acrylate functional group and a linker having a functional group polymerizable with the photocurable substance and an alkoxysilyl group,
- wherein the disc-shaped microparticle has a thickness of 5 μm to 300 μm and a diameter of 200 μm to 3,000 μm,
- wherein the polydopamine-coated surface layer is formed for a time period of 40 to 80 minutes,
- wherein the disc-shaped microparticle on which the polydopamine-coated surface layer is formed is configured to be cultured in three dimensions, and a density and shape of cells attached thereto can be observed by an inverted microscope, and
- wherein the disc-shaped microparticle for cell culture is optically transparent.
A method of preparing disc-shaped microparticles for cell culture having polydopamine-coated surfaces, the method comprising:
- (a) a step of preparing microparticles by photocuring polymerization of a mixture of a photocurable substance and a linker having a functional group polymerizable with the photocurable substance and an alkoxysilyl group;
- and
- (b) a step of adding a dopamine hydrochloride solution to the microparticles and stirring,
- wherein the stirring of step (b) is performed for a time period of 40 to 80 minutes,
- wherein the disc-shaped microparticle has a thickness of 5 μm to 300 μm and a diameter of 200 μm to 3,000 μm,
- wherein the disc-shaped microparticle on which the polydopamine-coated surface layer is formed is configured to be cultured in three dimensions, and a density and shape of cells attached thereto can be observed by an inverted microscope, and
- wherein the disc-shaped microparticle for cell culture is optically transparent.
Dependencies are inferred from the claim text.