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US 11,447,731 B2Granted
Three-dimensional bioreactors
AssigneeSouthwest Research Institute
InventorsJian Ling, Stephen T. Wellinghoff, Michael Joseph Rubal
Life of this patent
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Abstract
The present invention relates to the design, fabrication and applications of three-dimensional (3D) bioreactor for cell expansion and cell secreted substance production. The bioreactors have relatively low levels of potentially cytotoxic compounds, can be coated with substituted or unsubstituted poly(p-xylene) type coatings and can also be separately formed from liquid crystal photopolymerizable monomers.
Claims20
A 3D bioreactor for growth of cells comprising
- a plurality of voids having a surface area for cell expansion, said plurality of voids having a diameter D, a plurality of pore openings between said voids having a diameter d, such that D>d and wherein:
- (a) 90% or more of said voids have a selected void volume (V) that does not vary by more than +/−10.0%;
- and (b) 90% or more of said pore openings between said voids have a value of d that does not vary by more than +/−10.0%;
- wherein said bioreactor has a coating of a substituted or unsubstituted poly(p-xylylene) and polydopamine is applied to said poly(p-xylylene) coating wherein said polydopamine is capable of binding an additional layer or layers for cell culturing.
1 more element
A method of forming a 3D bioreactor comprising:
- forming said 3D bioreactor by additive manufacturing wherein said 3D bioreactor includes a plurality of voids having a surface area for cell expansion, said plurality of voids having a diameter D, a plurality of pore openings between said voids having a diameter d, such that D>d and wherein:
- (a) 90% or more of said voids have a selected void volume (V) that does not vary by more than +/−10.0%;
- and (b) 90% or more of said pore openings between said voids have a value of d that does not vary by more than +/−10.0%;
- and
- coating said bioreactor with substituted or unsubstituted poly(p-xylylene) and applying polydopamine to said poly(p-xylylene) coating wherein said polydopamine is capable of binding an additional layer or layers for cell culturing.
2 more elements
Dependencies are inferred from the claim text.