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US 11,447,729 B2Granted
Apparatus and method for bioprocessing
AssigneeGlobal Life Sciences Solutions USA LLC
InventorMark Robert Timmins
Life of this patent
Estimating the term…
Abstract
A bioreactor vessel incudes a first compartment configured to receive a suspension comprising cells and a cell culture medium, for use in a cell processing operation, a second compartment for receiving an overflow of the cell culture medium from the first compartment, and an overflow separating the first compartment from the second compartment, the overflow being configured to maintain a level of the cell culture medium in the first compartment.
Claims16
A bioreactor vessel, comprising:
- a bottom;
- a top;
- a plurality of sidewalls connecting the bottom with the top to define an interior chamber therein;
- a first compartment, disposed in the interior chamber, configured to receive a suspension comprising cells and a cell culture medium, for use in a cell processing operation, wherein the first compartment abuts each of the plurality of sidewalls;
- a second compartment, disposed in the interior chamber about the first compartment, for receiving an overflow of used cell culture medium from the first compartment, wherein the second compartment is surrounded entirely by the first compartment and spaced apart from the plurality of sidewalls;
- an inlet in fluid communication with the interior chamber to receive fresh cell culture medium that is supplied to the first compartment;
- an overflow separating the first compartment and the second compartment, the overflow configured to allow for passage of the used cell culture medium from the first compartment to the second compartment when the level of cell culture medium within the first compartment exceeds a height of the overflow to maintain a level of the cell culture medium in the first compartment at a height equal to or less than the height of the overflow, the amount of used cell culture medium passing over the overflow from the first compartment into the second compartment corresponds to the amount of fresh cell culture medium supplied to the first compartment from the inlet, wherein a volume of fresh cell culture medium added to the first compartment at a predetermined rate causes an equivalent volume of used cell culture medium to pass over the overflow into the second compartment at the same predetermined rate, maintaining a constant volume of cell culture medium in the first compartment;
- and
- an outlet associated with the second compartment and configured to receive the used cell culture medium passing over the overflow for passage out of the bioreactor vessel;
- wherein the height of the overflow is selectively adjustable to allow for adjustment of a depth of the suspension within the first compartment.
7 more elements
A bioreactor vessel, comprising:
- a bottom;
- a top;
- a plurality of sidewalls connecting the bottom with the top to define an interior chamber therein;
- a first compartment, disposed in the interior chamber, configured to receive a suspension comprising cells and a cell culture medium, for use in a cell processing operation;
- a second compartment, disposed in the interior chamber about the first compartment, for receiving an overflow of used cell culture medium from the first compartment;
- an overflow separating the first compartment and the second compartment, the overflow being configured to allow for passage of the used cell culture medium from the first compartment to the second compartment when the level of cell culture medium within the first compartment exceeds a height of the overflow to maintain a level of the cell culture medium in the first compartment at a height equal to or less than the height of the overflow;
- and
- an outlet associated with the second compartment and configured to receive the cell culture medium passing over the overflow and to allow for passage of the cell culture medium passing over the overflow out of the bioreactor vessel;
- wherein the overflow includes a plurality of vertically-spaced outlets extending between the top and the bottom, each outlet having a control valve associated therewith for selectively preventing or allowing a flow of the cell culture medium out of said outlet.
6 more elements
Dependencies are inferred from the claim text.