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US 11,447,582 B2Granted
Polymers and DNA copolymer coatings
AssigneeIllumina Cambridge Limited
InventorsAndrew A. Brown, Wayne N. George, Alexandre Richez, Anne-Cecile Dingwall, Xavier von Hatten
Life of this patent
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Abstract
Some embodiments described herein relate to new polymer coatings for surface functionalization and new processes for grafting pre-grafted DNA-copolymers to surface(s) of substrates for use in DNA sequencing and other diagnostic applications.
Claims21
A polymer for surface functionalization, comprising a recurring unit of Formula (V):
- wherein
- each R1d and R2d is independently hydrogen, optionally substituted alkyl or optionally substituted phenyl;
- R3d is hydrogen, optionally substituted alkyl, optionally substituted phenyl, or optionally substituted C7-14 aralkyl;
- RB is azido, substituted amino, Boc-protected amino, hydroxy, thiol, alkynyl, alkenyl, halo, epoxy, tetrazinyl, or aldehyde;
- L4 is an optionally substituted alkylene linker or an optionally substituted heteroalkylene linker;
- and
- L5 is
- and wherein n is an integer of 1 to 50.
5 more elements
A method for immobilizing a polymer to a first surface of a substrate, comprising:
- contacting a polymer comprising a recurring unit of Formula (V) with the first surface of the substrate, said first surface comprising a first plurality of functional groups covalently attached thereto;
- and
- reacting the first plurality of functional groups of the first surface with the polymer, thereby covalently bonding the polymer to the first surface of the substrate;
- wherein
- each R1d and R2d is independently hydrogen, optionally substituted alkyl or optionally substituted phenyl;
- R3d is hydrogen, optionally substituted alkyl, optionally substituted phenyl, or optionally substituted C7-14 aralkyl;
- RB is azido, substituted amino, Boc-protected amino, hydroxy, thiol, alkynyl, alkenyl, halo, epoxy, tetrazinyl, or aldehyde;
- L4 is an optionally substituted alkylene linker or an optionally substituted heteroalkylene linker;
- and
- L5 is
- and wherein n is an integer of 1 to 50.
8 more elements
Dependencies are inferred from the claim text.