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US 11,447,547 B1Granted
Method of antigen-binding protein production
AssigneeAmgen Inc.
InventorsElaheh Binabaji, Chio Mui Chan, Hadley Krizner, Junfen Ma, Diana Woehle
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Abstract
The present disclosure relates to methods of re-oxidizing an antigen-binding protein.
Claims35
A method of re-oxidizing a therapeutic protein comprising at least one di-sulfide bridge, said method comprising:
- (a) subjecting a load composition comprising the therapeutic protein to an affinity chromatography column or membrane;
- and
- (b) washing the affinity chromatography column or membrane, wherein the wash comprises a solution comprising copper at a concentration ranging from 2 μM to 50 μM to produce a flow-through composition,
- wherein a decreased level of partially-reduced therapeutic protein species is present in the flow-through composition compared to the load composition.
1 more element
A method of re-oxidizing a therapeutic protein comprising at least one reduced di-sulfide bridge, said method comprising:
- (a) incubating a load composition comprising the therapeutic protein at a temperature of approximately 2 to 8° C.;
- (b) subjecting the load composition to an affinity chromatography column or membrane;
- and
- (c) washing the affinity chromatography column or membrane, wherein the wash comprises a solution comprising copper in an amount ranging from 2 μM to 50 μM to produce a flow-through composition,
- wherein a decreased level of partially-reduced therapeutic protein species is present in the flow-through composition compared to the load composition.
2 more elements
Dependencies are inferred from the claim text.