Fibronectin based scaffold proteins
AssigneeBristol-Myers Squibb Company
InventorsDasa Lipovsek, Jonathan H. Davis
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Abstract
Fibronectin type III (10Fn3) binding domains having novel designs that are associated with reduced immunogenicity are provided. The application describes alternative 10Fn3 binding domains in which certain immunogenic regions are not modified when producing a binder in order to maintain recognition as a self antigen by the host organism. The application also describes 10Fn3 binding domains in which HLA anchor regions have been destroyed thereby reducing the immunogenic contribution of the adjoining region. Also provided are 10Fn3 domains having novel combinations of modified regions that can bind to a desired target with high affinity.
Claims8
A library consisting of a plurality of polypeptides, wherein each of the polypeptides in the library comprises a 10Fn3 domain comprising an amino acid sequence selected from the group consisting of SEQ ID NOs:
- 76-87, 118-120 and 128-131, wherein at least 50% of amino acids x are not the amino acid at the corresponding position in SEQ ID NO:
- 1 or 6.
Dependencies are inferred from the claim text.