Compositions and methods for enhanced production of enduracidin in a genetically engineered strain of streptomyces fungicidicus
AssigneeOregon State University
InventorsT. Mark Zabriskie, Xihou Yin
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Abstract
This disclosure describes compositions and methods for enhanced production of enduracidin in genetically engineered strains of Streptomyces fungicidicus. In particular, the present disclosure describes the genetic manipulation of regulatory genes orf24 and orf18 associated with the enduracidin (enramycin) biosynthesis gene cluster from Streptomyces fungicidicus to generate vector constructs and recombinant strains producing greater yields of enduracidin.
Claims17
A recombinant strain of Streptomyces fungicidicus comprising one or more modified genes selected from the group consisting of an augmented open reading frame-24 (orf24) that encodes the amino acid sequence of SEQ ID NO:
- 26, and a diminished open reading frame-18 (orf18), wherein an enhanced production of enduracidin is obtained with the recombinant strain of Streptomyces fungicidicus in comparison to that obtained with a control Streptomyces fungicidicus strain.
The recombinant strain of Streptomyces fungicidicus that is BM38-2.24/16 (ATCC Deposit No. PTA-124006).
An expression vector selected from the group consisting of pXY152-endorf24 (SEQ ID NO:3), pXY152-endorf24-camtsr (SEQ ID NO:
- 20), and pXY152-endorf24-blatsr (SEQ ID NO:
- 23).
Dependencies are inferred from the claim text.