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US 11,447,528 B2Granted
Method for extracting hepatitis a virus (HAV) antigen from cell culture
AssigneeMediagnost Gesellschaft für Forschung und Herctellung von Diagnostika GmbH
InventorBertram Flehmig
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Abstract
The present invention is directed to a method for extracting hepatitis A virus (HAV) antigen from a cell culture as well as a use of a composition for extracting hepatitis A virus (HAV) antigen from a cell culture.
Claims12
A method for extracting hepatitis A virus (HAV) antigen from a cell culture, the method comprising the steps of:
- a) providing at least one mammalian cell expressing a nucleic acid coding for a hepatitis A virus (HAV) antigen,
- b) lysing the at least one mammalian cell expressing the nucleic acid coding for said HAV antigen by contacting the cells, which are attached to a cell culture support, with at least one protease and at least one surfactant so that the at least one protease and the at least one surfactant act on the at least one mammalian cell together for a sufficient period of time during lysing, and
- c) collecting a lysate comprising said HAV antigen,
- wherein said HAV antigen comprises at least one of a mature VP1 capsid protein of hepatitis A virus, a mature VP2 capsid protein of hepatitis A virus, a mature VP3 capsid protein of hepatitis A virus, or mixtures thereof.
1 more element
Dependencies are inferred from the claim text.