Wound healing and tissue engineering
AssigneesThe Brigham and Women's Hospital, Inc., Children's Medical Center Corporation, The United States Government as represented by the Department of Veterans Affairs
InventorsMarkus H. Frank, Natasha Y. Frank, Dennis P. Orgill, George F. Murphy
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Abstract
The present invention is directed to wound healing scaffolds cografted with a population of stem cells, wherein the population of stem cells are ABCB5+ stem cells. The scaffolds are, for instance, collagen glycosaminoglycan scaffolds.
Claims10
A method for tissue engineering, comprising seeding a biological tissue scaffold with ABCB5(+) stem cells, maintaining the scaffold under conditions such that tissue is formed, and implanting the seeded scaffold at a site of a tissue defect,
- wherein the scaffold is a porous matrix of cross-linked collagen and glycosaminoglycan and wherein the scaffold includes a separate semi-permeable layer,
- wherein at least 99% of the total cells present in the scaffold are ABCB5(+) stem cells, and
- wherein the ABCB5(+) stem cells are not treated with a soluble cytokine ex-vivo or in vitro prior to implantation.
Dependencies are inferred from the claim text.