Pumpkin seed protein nanoparticles, methods for preparing and using thereof

A method for preparing a high internal phase emulsion of pumpkin seed protein nanoparticles, comprising:

• dissolving pumpkin seed protein powders in water and adjusting pH to approximately 8 to 11 to obtain a pumpkin seed protein solution;
• adding the pumpkin seed protein solution through a peristaltic pump into an ethanol solution with stiffing for 4 hours to obtain a first solution;
• performing a centrifugation to the first solution and collecting precipitates;
• diluting the precipitates with water and performing a freeze-drying to obtain the pumpkin seed protein nanoparticles;
• dispersing the pumpkin seed protein nanoparticles in water in an amount of approximately 1.5 to 2% w/v to obtain a pumpkin seed protein nanoparticles solution;
• adding an essence to the pumpkin seed protein nanoparticles solution in an amount of approximately from 80 to 82% w/v, maintaining pH at approximately 3, 8 or 9 and performing a high speed shearing under 12000 rpm in a homogenizer to obtain a high internal phase emulsion containing the essence;
• and
• wherein the concentration of the pumpkin seed protein in the pumpkin seed protein solution is approximately from 5 to 15 mg/ml;
• wherein the ethanol solution is an anhydrous ethanol solution and the mass ratio of the ethanol solution to the pumpkin seed protein solution is approximately 3:1.