Method and container for preserving corneal endothelial cells
AssigneeThe Doshisha
InventorsNoriko Koizumi, Naoki Okumura
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Abstract
The present disclosure provides a method of preserving corneal endothelial cells at a high cell survival rate. The present disclosure provides a method of preserving corneal endothelial cells and/or corneal endothelium-like cells, comprising preserving the corneal endothelial cells and corneal endothelium-like cells in a container with a bottom area of at least about 0.7 cm2. Accordingly to the present invention, corneal endothelial cells can be preserved at a high cell survival rate. Corneal endothelial cells preserved in this manner have functions of normal corneal endothelial cells. Such cells can also be used as cells for treating a corneal endothelial disease or the like.
Claims15
A method of preserving corneal endothelial cells and/or corneal endothelium-like cells in a cell suspension state that can be used in cell injection therapy, comprising preserving the corneal endothelial cells and/or corneal endothelium-like cells in a container with a bottom area of at least about 0.7 cm2, wherein a height of a liquid surface of a liquid for preserving the cells is about 0.75 mm or greater, and wherein the corneal endothelial cells and/or corneal endothelium-like cells are preserved for about 24 to 72 hours, wherein the preserved corneal endothelial cells and corneal endothelium-like cells are characterized by being administered without additional processing or culturing.
Dependencies are inferred from the claim text.