Quantitative trait loci (QTL) associated with shatter resistant capsules in sesame and uses thereof
AssigneeEqui-Nom Ltd.
InventorsOron Gar, Arie Zackay, Gil Shalev
Life of this patent
Estimating the term…
Abstract
The invention relates to sesame genetics and breeding. Specifically, the invention relates to genetic improvement for shatter resistant capsules. More specifically, the invention relates to novel quantitative trait loci (QTL) conferring shatter resistant capsules, and methods thereof, including methods for introgressing the novel QTL into elite germplasm in a breeding program for shatter resistant capsules.
Claims17
A sesame plant, progeny thereof or part thereof having shatter-resistant capsules, the sesame plant, progeny thereof or part thereof comprising at least three shatter-resistant capsule loci, wherein at least one shatter-resistant capsule locus is from at least one different sesame variety, wherein the at least three shatter-resistant capsule loci are associated with at least three quantitative trait loci (QTL) selected from QTL 1, QTL 2, QTL 3, QTL 4, QTL5, QTL 6 and QTL 7, wherein each of QTLs 1-7 is linked to at least one nucleic acid genetic marker that is associated with shatter resistance of the capsules, wherein the at least three QTLs comprise at least three of:
- QTL 1 linked to genetic marker LG3_19205572, wherein LG3_19205572 has the nucleic acid sequence set forth in SEQ ID NO:
- 1 or 9, wherein QTL 1 is homozygous or heterozygous with respect to SEQ ID NO:
- 1,
- QTL 2 linked to genetic marker LG5_12832234, wherein LG5_12832234 has the nucleic acid sequence set forth in SEQ ID NO:
- 2 or 10, wherein QTL 2 is homozygous or heterozygous with respect to SEQ ID NO:
- 2,
- QTL 3 linked to genetic marker LG6_2739268, wherein LG6_2739268 has the nucleic acid sequence set forth in SEQ ID NO:
- 3 or 11, wherein QTL 3 is homozygous or heterozygous with respect to SEQ ID NO:
- 3,
- QTL 4 linked to genetic marker LG7_5141423, wherein LG7_5141423 has the nucleic acid sequence set forth in SEQ ID NO:
- 4 or 12, wherein QTL 4 is homozygous or heterozygous with respect to SEQ ID NO:
- 4,
- QTL 5 linked to genetic marker LG11_8864255, wherein LG11_8864255 has the nucleic acid sequence set forth in SEQ ID NO:
- 5 or 13, wherein QTL 5 is homozygous or heterozygous with respect to SEQ ID NO:
- 5,
- QTL 6 linked to genetic markers LG15_4900868 and LG15_5315334, wherein LG15_4900868 has the nucleic acid sequence set forth in SEQ ID NO:
- 6 or 14 and LG15_5315334 has the nucleic acid sequence set forth in SEQ ID NO:
- 7 or 15, wherein QTL 6 is homozygous or heterozygous with respect to SEQ ID NO:
- 6 and is homozygous or heterozygous with respect to SEQ ID NO:
- 7, and
- QTL 7 linked to genetic marker LG16_1563304, wherein LG16_1563304 has the nucleic acid sequence set forth in SEQ ID NO:
- 8 or 16, wherein QTL 7 is homozygous or heterozygous with respect to SEQ ID NO:
- 8,
- wherein the genetic markers are arranged in one or more marker cassettes, wherein:
- marker cassette 1 comprises LG3_19205572, LG7_5141423 and LG15_5315334, wherein the alleles for said LG3_19205572, LG7_5141423 and LG15_5315334 are homozygous or heterozygous,
- marker cassette 2 comprises LG3_19205572, LG11_8864255 and LG15_5315334, wherein the alleles for said LG3_19205572, LG11_8864255 and LG15_5315334 are homozygous or heterozygous,
- marker cassette 3 comprises LG3_19205572, LG5_12832234, LG15_4900868 and LG15_5315334, wherein the alleles for said LG3_19205572, LG5_12832234, LG15_4900868 and LG15_5315334 are homozygous or heterozygous, and
- marker cassette 4 comprises LG6_2739268, LG11_8864255 and LG16_1563304, wherein the alleles for said LG6_2739268, LG11_8864255 and LG16_1563304 are homozygous or heterozygous.
A sesame plant, progeny thereof or part thereof having shatter-resistant capsules, the sesame plant, progeny thereof or part thereof comprising three shatter-resistant capsule loci, wherein at least one shatter-resistant capsule locus is from at least one different sesame variety, wherein each of the three shatter-resistant capsule loci is associated with a quantitative trait loci (QTL), wherein the QTLs are QTL 1, QTL 4 and QTL 6, and each QTL is linked to at least one nucleic acid genetic marker that is associated with shatter resistance of the capsules, wherein each nucleic acid genetic marker is arranged in a marker cassette 1 comprising:
- QTL 1 linked to genetic marker LG3_19205572, wherein LG3_19205572 has the nucleic acid sequence set forth in SEQ ID NO:
- 1 or 9;
- QTL 4 linked to genetic marker LG7_5141423, wherein LG7_5141423 has the nucleic acid sequence set forth in SEQ ID NO:
- 4 or 12;
- and
- QTL 6 linked to genetic marker LG15_5315334, wherein LG15_5315334 has the nucleic acid sequence set forth in SEQ ID NO:
- 7 or 15,
- wherein alleles for said LG3_19205572, LG7_5141423, and LG15_5315334 are homozygous or heterozygous.
A sesame plant, progeny thereof or part thereof having shatter-resistant capsules, the sesame plant, progeny thereof or part thereof comprising three shatter-resistant capsule loci, wherein at least one shatter-resistant capsule locus is from at least one different sesame variety, wherein at each of the three shatter-resistant capsule loci is associated with a quantitative trait loci (QTL), wherein the QTLs are QTL 1, QTL 2, and QTL 6, and each QTL is linked to at least one nucleic acid genetic marker that is associated with shatter resistance of the capsules, wherein each nucleic acid genetic marker is arranged in a marker cassette 3 comprising:
- QTL 1 linked to genetic marker LG3_19205572, wherein LG3_19205572 has the nucleic acid sequence set forth in SEQ ID NO:
- 1 or 9;
- QTL 2 linked to genetic marker LG5_12832234, wherein LG5_12832234 has the nucleic acid sequence set forth in SEQ ID NO:
- 2 or 10,
- QTL 6 linked to genetic markers LG15_4900868 and LG15_5315334, wherein LG15_4900868 has the nucleic acid sequence set forth in SEQ ID. NO:
- 6 or 14 and LG15_5315334 has the nucleic acid sequence set forth in SEQ ID NO:
- 7 or 15,
- wherein alleles for said LG3_19205572, LG5_12832234, LG15_4900868 and LG15_5315334 are homozygous or heterozygous.
Dependencies are inferred from the claim text.