# US 11,442,258 B2 · Compact optical system for substantially simultaneous monitoring of samples in a sample array

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- Number: US 11,442,258 B2
- Document ID: US11442258B2
- Status: Granted
- Kind: B2
- Application number: 16/909,146
- Filed: 2020-06-23
- Published: 2020-10-08
- Granted: 2022-09-13
- Earliest claimed: 2013-03-15
- Assignees: BioFire Defense, LLC
- Applicants: BioFire Defense, LLC
- Inventors: Richard David Abbott; Patrick L. Riley; Zackery Kent Evans; Lyle M. Nay
- Web page: https://patentgrep.com/patent/US11442258B2

## CPC

- G02B 17/06: using mirrors only {, i.e. having only one curved mirror (used in non-imaging applications G02B19/00)}
- B01L 7/52: {with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples}
- G02B 13/22: Telecentric objectives or lens systems
- G02B 25/007: {comprising other optical elements than lenses (G02B25/004, G02B25/005 take precedence)}
- G02B 27/0025: {for optical correction, e.g. distorsion, aberration}
- B01L 2300/0654: Lenses; Optical fibres
- B01L 2300/0829: Multi-well plates; Microtitration plates
- B01L 2300/1816: using induction heating
- B01L 2300/1822: using Peltier elements
- B01L 2300/1827: using resistive heater
- B01L 2300/1844: using fans
- B01L 2300/1861: using radiation
- G02B 21/34: Microscope slides, e.g. mounting specimens on microscope slides
- G02B: OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE

## Abstract

Optical systems and apparatuses configured for enabling substantially simultaneous observation of a plurality of points in an array from a common reference point. Without the optical systems and apparatuses disclosed herein, less than all of the plurality of points can be observed substantially simultaneously from the common reference point.

## Claims

### Claim 1

An apparatus, comprising:
a sample block positioned at a first end of a body, the sample block having a plurality of recessed receptacles, each recessed receptacle sized and shaped to receive a sample container,
an observation element positioned at a second end of the body, the observation element having a light source and a camera, the light source positioned to transmit light toward the first end of the body,
a reflective element positioned above the sample block at the first end of the body, the reflective element having a single reflective surface formed as a non-apex portion of a paraboloid, wherein the light source is positioned relative to the reflective element such that the reflective element directs light received from the light source simultaneously to each sample container and reflects an image of each sample container simultaneously to the observation element, wherein the reflected image of each sample container is distorted to form a fan shaped image that yields a first data set that represents the distorted image, the first data set comprising a fluorescent signal for each sample container, and
a computer programmed to communicate with the observation element and to process the first set of data such that the first set of data is processed to yield a second set of data that represents an undistorted image.

### Claim 2 (depends on 1)

The apparatus of claim 1, further comprising a thermocycler.

### Claim 3 (depends on 1)

The apparatus of claim 1, wherein the light source includes at least two lamps configured to produce at least six different excitation wavelengths.

### Claim 4 (depends on 1)

The apparatus of claim 1, wherein the sample block includes a multi-well plate having at least 96 sample wells.

### Claim 5 (depends on 1)

The apparatus of claim 1, wherein the light source includes a bandpass filter configured to pass at least to two different bands of light from the light source to excite two or more fluorophores within the sample container to emit a fluorescent signal.

### Claim 6 (depends on 1)

The apparatus of claim 1, wherein the camera includes one or more filters configured to pass fluorescent signals emitted from a sample within the sample container, each fluorescent signal being in a range of 1 to 4 different wavelengths.

### Claim 7 (depends on 1)

The apparatus of claim 1, wherein one or more of the camera or the light source includes at least one filter selected from the group consisting of a 510 nm filter, a 555 nm filter, a 620 nm filter, and a 710 nm filter.