# US 11,442,117 B2 · Systems, devices, and methods for electroporation induced by magnetic fields > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 11,442,117 B2 - Document ID: US11442117B2 - Status: Granted - Kind: B2 - Application number: 16/722,520 - Filed: 2019-12-20 - Published: 2020-12-03 - Granted: 2022-09-13 - Earliest claimed: 2016-11-09 - Assignees: Sigma Genetics, Inc. - Applicants: Sigma Genetics, Inc. - Inventors: Christopher James Pihl; Matthew Rein Scholz; David Edwin Kirtley - Web page: https://patentgrep.com/patent/US11442117B2 ## CPC - C12N 13/00: Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves - G01R 33/0023: {Electronic aspects, e.g. circuits for stimulation, evaluation, control; Treating the measured signals; calibration (G01R33/0017 takes precedence)} - A61B 5/05: Detecting, measuring or recording for diagnosis by means of electric currents or magnetic fields; Measuring using microwaves or radio waves (measuring movement of the entire body or parts thereof A61B5/11; detecting, measuring or recording bioelectric or biomagnetic signals of the body or parts thereof A61B5/24) - A61K 41/0023: {Aggression treatment or altering} - A61N 1/40: Applying electric fields by inductive or capacitive coupling (microwave apparatus A61N5/00); {Applying radio-frequency signals} - C12N 15/87: Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation - A61M 2037/0007: {having means for enhancing the permeation of substances through the epidermis, e.g. using suction or depression, electric or magnetic fields, sound waves or chemical agents} - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) - G01R: MEASURING ELECTRIC VARIABLES; MEASURING MAGNETIC VARIABLES (indicating correct tuning of resonant circuits H03J3/12) - A61B: DIAGNOSIS; SURGERY; IDENTIFICATION (usefulness limited to only animals A61D) - A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D) - A61N: ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY (measurement of bioelectric currents A61B; surgical instruments, devices or methods for transferring non-mechanical forms of energy to or from the body A61B18/00; anaesthetic apparatus in general A61M; incandescent lamps H01K; infrared radiators for heating H05B) - A61M: DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY (introducing media into or onto the bodies of animals A61D7/00; means for inserting tampons A61F13/26; devices for administering food or medicines orally A61J; containers for collecting, storing or administering blood or medical fluids A61J1/05); DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY (surgery A61B; chemical aspects of surgical articles A61L); DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR {(Electrotherapy, e.g. producing anaesthesia by the use of alternating or intermittent currents A61N1/36021)} ## Abstract A system includes a control device and a magnetic device coupled to the control device. The magnetic device is configured to inductively couple to a treatment target including one or more cells exposed to an agent, and includes one or more magnetic coils. The control device and the magnetic device are collectively configured to generate and apply a transient magnetic field to the treatment target to induce an electric field and porate the one or more cells and to permit the agent to enter the one or more cells. ## Claims ### Claim 1 A system, comprising: a control device including a set of independent circuits; a set of magnetic devices coupled to the control device, the set of magnetic devices configured to magnetically or inductively couple to a treatment target including one or more cells exposed to an agent, each magnetic device of the set of magnetic devices including one or more magnetic coils and being independently addressable by a corresponding circuit of the set of independent circuits; and a user interface configured to receive an indication of spatial information associated with an application of a set of transient magnetic fields, the control device and the set of magnetic devices collectively configured to generate and apply the set of transient magnetic fields based on the indication of spatial information to the treatment target to porate the one or more cells and to permit the agent to enter the one or more cells. ### Claim 2 (depends on 1) The system of claim 1, the set of transient magnetic fields configured to induce an electric field in the treatment target to porate the one or more cells, wherein the one or more magnetic coils of the set of magnetic devices are configured in proximity to at least a portion of the treatment target during use. ### Claim 3 (depends on 1) The system of claim 1, the control device including a signal generator configured to apply an electrical signal to each magnetic device of the set of magnetic devices, the signal generator selected from the group consisting of an oscillator, a frequency synthesizer, a sine-wave generator, a pulse generator, a random noise generator, an arbitrary waveform generator, and combinations thereof, the electrical signal selected from the group consisting of a pulsed ¼ sine wave, followed by an L/R decay, a sine wave, a decaying sine wave, a square wave, and an arbitrary waveform. ### Claim 4 (depends on 1) The system of claim 1, wherein each magnetic device of the set of0 magnetic devices includes: a ferromagnetic circuit element as an inductor, or a superconducting electromagnet configured to generate a transient magnetic field of the set of transient magnetic fields. ### Claim 5 (depends on 1) The system of claim 1, wherein the one or more magnetic coils of the set of magnetic devices are one or more oscillating antennae, wherein each oscillating antenna of the one or more oscillating antennae is independently addressable. ### Claim 6 (depends on 1) The system of claim 1, wherein the set of transient magnetic fields defines a treatment volume of at least about 10 μL. ### Claim 7 (depends on 1) The system of claim 1, wherein the set of transient magnetic fields is configured to reversibly or irreversibly porate the one or more cells to permit the agent to enter the one or more cells. ### Claim 8 (depends on 1) The system of claim 1, wherein the treatment target includes at least one of: a container, a medical device, or a mammalian target, wherein the one or more cells are in vitro, in vivo, or ex vivo. ### Claim 9 (depends on 1) The system of claim 1, wherein the one or more cells include at least one of: mammalian cells selected from the group consisting of heart cells, liver cells, kidney cells, skin cells, brain cells, bladder cells, testes cells, ovary cells, uterus cells, eye cells, pancreatic cells, fallopian tube cells, vaginal cells, teste cells, prostate cells, placenta cells, large intestine cells, small intestine cells, colon cells, cancer cells, muscle cells, epithelial cells, connective tissue cells, nerve cells, blood cells, white blood cells, red blood cells, T cells, B cells, lymphocytes, antigen presenting cells, platelets, macrophages, monocytes, granulocytes neutrophils, eosinophils, basophils, and cancer cells; bacterial cells; fungal cells; parasite cells; or plant cells. ### Claim 10 (depends on 1) The system of claim 1, wherein the agent is a nucleic acid. ### Claim 11 (depends on 1) The system of claim 1, wherein the agent is a polypeptide, wherein the polypeptide is selected from the group consisting of an enzyme, a ligand, a receptor, a structural protein, a fusion protein, an antigenic polypeptide, an antigen-binding moiety, a ligand-receptor fusion, an antibody, and a fragment thereof. wherein the antigenic polypeptide is from a mammalian cell, a bacteria, virus, parasite, or fungus, wherein the antibody or fragment thereof is selected from the group consisting of a monoclonal antibody, polyclonal antibody, chimeric antibody, humanized antibody, scFv, VL, VH, CL, CH1 domain, F(ab)2 fragment, a bivalent antibody, a Fd fragment, or a Fv fragment. ### Claim 12 (depends on 1) The system of claim 1, wherein the agent is a conjugated drug or molecule that is radioimmunotherapy (RIT), antibody-directed enzyme prodrug therapy (ADEPT) or an antibody-drug conjugate. ### Claim 13 (depends on 1) The system of claim 1, wherein the agent is a small molecule drug. ### Claim 14 A method, comprising: exposing one or more cells in a treatment target to an agent; receiving an indication of spatial information associated with an application of a set of transient magnetic field; and applying the set of transient magnetic fields to the treatment target based on the indication of spatial information to porate the one or more cells and to permit the agent to enter the one or more cells. ### Claim 15 (depends on 14) The method of claim 14, the transient magnetic fields configured to induce an electric field in the treatment target to porate the one or more cells. ### Claim 16 (depends on 14) The method of claim 14, further comprising generating the set of transient magnetic fields by applying an electrical signal to a magnetic device that is inductively coupled to the treatment target.