# US 11,442,055 B2 · Method and system for detecting an analyte present in a liquid specimen

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- Number: US 11,442,055 B2
- Document ID: US11442055B2
- Status: Granted
- Kind: B2
- Application number: 16/931,535
- Filed: 2020-07-17
- Published: 2021-01-21
- Granted: 2022-09-13
- Earliest claimed: 2019-07-17
- Assignees: Commissariat A L'energie Atomique Et Aux Energies Alternatives
- Applicants: Commissariat a I'Energie Atomique et aux Energies Alternatives
- Inventors: Myriam-Laure Cubizolles; Thomas Bordy; Frederic Revol-Cavalier
- Web page: https://patentgrep.com/patent/US11442055B2

## CPC

- G01N 33/492: {Determining multiple analytes}
- B01L 3/502715: {characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces}
- G01N 1/08: involving an extracting tool, e.g. core bit
- G01N 1/38: Diluting, dispersing or mixing samples
- G01N 21/49: within a body or fluid
- G01N 33/48785: {Electrical and electronic details of measuring devices for physical analysis of liquid biological material not specific to a particular test method, e.g. user interface or power supply}
- G01N 33/5304: {Reaction vessels, e.g. agglutination plates (for solid-phase systems G01N33/543)}
- G01N 33/54313: {the carrier being characterised by its particulate form}
- G01N 33/57535: of the large intestine, e.g. colon, rectum or anus
- G01N 33/726: {Devices}
- B01L 2200/027: for microfluidic devices
- B01L 2300/044: pierceable, e.g. films, membranes
- B01L 2300/0672: Integrated piercing tool
- B01L 2300/0681: Filter
- B01L 2300/0816: Cards, e.g. flat sample carriers usually with flow in two horizontal directions
- B01L 2300/0867: Multiple inlets and one sample wells, e.g. mixing, dilution
- B01L 2400/049: vacuum
- G01N 33/721: {Haemoglobin}
- G01N 2001/2873: {Cutting or cleaving}
- G01N 2001/288: {Filter punches}
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE

## Abstract

The invention relates to a method for detecting an analyte present in a liquid specimen, including: injecting the liquid specimen into a detection chamber, the detection chamber having a non-zero volume enclosing polymeric beads covered with a reagent suitable for the analyte to be detected; capturing at least one image of at least one region of the detection chamber using a sensor; processing the image acquired by the sensor, which includes determining a texture level of the acquired image; and determining a concentration of the analyte depending on the texture level determined for the image.

## Claims

### Claim 1

A system for detecting an analyte present in a liquid specimen, comprising:
a medium equipped with a fluidic circuit, said fluidic circuit comprising at least one injection channel and a detection chamber into which said injection channel opens, said detection chamber having a non-zero volume enclosing polymeric beads covered with a reagent suitable for said analyte to be detected,
a preparing device for preparing the liquid specimen to be analyzed, said medium being fitted on said preparing device, said preparing device comprising a coring implement for coring a sample of material and a dissolving or re-suspending implement for dissolving or re-suspending each core produced by said coring implement, said injection channel of the fluidic circuit being connected to said dissolving or re-suspending implement,
an optical read-out device, comprising an image sensor arranged to acquire an image of at least one region of the detection chamber, and
a processing unit configured to process the image acquired by the image sensor, comprising a module for determining a texture level of each acquired image and a module for determining a concentration of said analyte depending on the texture level determined for each image, said texture level depending on an agglutination level of the polymeric beads in the detection chamber in the presence of the analyte.

### Claim 2 (depends on 1)

The system according to claim 1, wherein the image sensor and the at least one region of the detection chamber are positioned in a defocused manner.

### Claim 3 (depends on 1)

The system according to claim 1, wherein the optical read-out device comprises a source of light radiation arranged to emit light radiation through the detection chamber, said image sensor being positioned on the other side of said source with respect to the at least one region of the detection chamber.

### Claim 4 (depends on 1)

The system according to claim 1, wherein the medium consists of a micro-fluidic card made from a transparent or semi-transparent material.

### Claim 5 (depends on 4)

The system according to claim 4, wherein the dissolving or re-suspending implement comprises one or more cuvettes each intended to receive a dissolving or re-suspending liquid.

### Claim 6 (depends on 5)

The system according to claim 5, wherein the preparing device comprises a body and in that the coring implement comprises one or more die-cutters that are movable with respect to the body, each die-cutter interacting translationally with said body in a main direction, between at least one rest state in which the die-cutter is plumb with a deposition surface intended to receive said sample of material and an actuated state in which the die-cutter is located inside a respective cuvette among the one or more cuvettes of the dissolution or re-suspension implement.

### Claim 7 (depends on 6)

The system according to claim 6, wherein each cuvette comprises an aperture and at least one perforable film covers the aperture of each cuvette.

### Claim 8 (depends on 1)

The system according to claim 1, wherein the fluidic circuit comprises a region for filtering particles present in the liquid specimen.

### Claim 9 (depends on 1)

The system according to claim 1, wherein the optical read-out device and the processing unit are grouped together in one and a same device of smartphone type.