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US 11,442,046 B2Granted
Reagent for mass spectrometry
AssigneeRoche Diagnostics Operations, Inc.
InventorsThomas Carell, Uwe Kobold, Dieter Heindl, Silvia Baecher, Andreas Leinenbach, Martin Rempt, Toni Pfaffeneder, Angie Kirchner, Olesea Kosmatchev, Rene Rahimoff, Sarah Schiffers, Markus Muller
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Abstract
The present invention relates to reagents suitable in the mass spectrometric determination of analyte molecules such as carbohydrates as well as adducts of such reagents and analyte molecules and applications of said reagents and adducts. Further, the present invention relates to methods for the mass spectrometric determination of analyte molecules.
Claims20
Use of a compound of the general formula (I):
- X-L1-Y(-L2-Z)r
- wherein
- X is a reactive group capable of reacting with an analyte molecule, whereby a covalent bond with the analyte molecule is formed,
- L1 is a bond or a spacer,
- Y is a neutral ion loss unit, which itself is neutral and which, under conditions of mass spectrometry, is capable of fragmentation, whereby a neutral species is released, and wherein Y comprises a 4-, 5-, or 6-membered heterocyclic moiety,
- L2 is a bond or a spacer,
- Z is a charge unit comprising
- (i) at least one positively charged moiety having a pKa of 10 or higher, or
- (ii) at least one negatively charged moiety having a pKb of 10 or higher,
- r is 1,
- including any salt thereof,
- or of a composition or kit comprising at least one compound (I)
- for the mass spectrometric determination of an analyte molecule.
10 more elements
A reagent, which is a compound of formula (Ia)
- X-L1-Y-L2-Z
- wherein
- X is a carbonyl reactive group, dienophilic group, a carboxylate reactive group, a phenol reactive group, an amino reactive group, a hydroxyl reactive group, or a thiol reactive group, and wherein X is no acrylester,
- L1 is a bond or a spacer,
- Y is a neutral ion loss unit, which itself is neutral and which, under conditions of mass spectrometry, is capable of fragmentation, whereby a neutral species is released, and wherein Y consists of a 4-, 5-, or 6-membered heterocyclic moiety,
- L2 is a bond or a spacer,
- Z is a charge unit comprising at least one permanently positively charged moiety selected from the group consisting of a primary, secondary, tertiary or quaternary ammonium group and a phosphonium group, wherein the overall molecule has a pKa of 10 or higher,
- including any salt thereof, or a composition or kit comprising at least one compound (Ia).
5 more elements
Dependencies are inferred from the claim text.