# US 11,441,975 B2 · Controlling method of preprocessing apparatus

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- Number: US 11,441,975 B2
- Document ID: US11441975B2
- Status: Granted
- Kind: B2
- Application number: 15/322,527
- Filed: 2014-07-02
- Published: 2017-05-18
- Granted: 2022-09-13
- Earliest claimed: 2014-07-02
- Assignees: Shimadzu Corporation
- Applicants: Shimadzu Corporation
- Inventors: Nobuhiro Hanafusa; Masamitsu Shikata
- Web page: https://patentgrep.com/patent/US11441975B2

## CPC

- G01N 1/10: in the liquid or fluent state {(burettes, pipettes B01L3/02; sampling of ground water E02D1/06; metering by volume of fluids or fluent solid material G01F11/00, G01F13/00)}
- B01L 3/502: {with fluid transport, e.g. in multi-compartment structures}
- G01N 1/14: Suction devices, e.g. pumps; Ejector devices
- G01N 1/34: Purifying; Cleaning {(processes or apparatus for extracting or separating nucleic acids from biological samples C12N15/1003)}
- G01N 1/4005: {by transferring a selected component through a membrane}
- G01N 30/06: Preparation
- G01N 35/0099: {comprising robots or similar manipulators (robots per se B25J)}
- G01N 35/025: {having a carousel or turntable for reaction cells or cuvettes}
- B01L 2200/0631: Purification arrangements, e.g. solid phase extraction [SPE]
- G01N 2001/4011: {being a ion-exchange membrane}
- G01N 2035/00485: {combined with sample carriers}
- G01N 2035/00534: {Mixing by a special element, e.g. stirrer}
- G01N 2035/0427: {nestable or stockable}
- G01N 2035/0441: {for samples}
- G01N 2035/0443: {for reagents}
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE

## Abstract

A preprocessing apparatus uses preprocessing kits prepared for samples, respectively, sets the preprocessing kits in plural processing ports provided so as to correspond to preprocessing items, and executes a predetermined preprocessing item. A controller which controls operations of a carrying mechanism holding and carrying the preprocessing kits, and processing parts executing the preprocessing items in the respective processing ports, includes a processing-state control part, a random access part, and a preprocessing part. The random access part is configured to check availability of a preprocessing port corresponding to a preprocessing item to be executed on a sample contained in a preprocessing kit, and set the preprocessing kit in a processing port if the processing port is available as a processing port corresponding to the preprocessing item. The preprocessing part executes a corresponding preprocessing item on a sample in a preprocessing kit when the preprocessing kit is set in the processing port.

## Claims

### Claim 1

A controlling method of a preprocessing apparatus for preprocessing of one or more samples in preparation for sample analysis, wherein
the preprocessing apparatus comprises:
a preprocessing container which is prepared for each sample of the one or more samples and includes a separation device and a collecting container,
a carrying mechanism, which includes a holding part configured to hold the separation device and the collecting container of the preprocessing container individually, the carrying mechanism carrying the separation device and the collecting container individually by moving the holding part;
a preprocessing-container setting part configured to set the preprocessing container in a position on a path of the holding part;
a plurality of stirring ports, each having a stirring mechanism, configured to receive the separation device from the holding part of the carrying mechanism and provided for executing a stirring processing to the separation device,
a plurality of filtration ports configured to receive the collecting device from the holding part of the carrying mechanism and provided for executing a filtration processing which is a process to extract the sample from the separation device which is set on the collecting container to the collecting container,
wherein the separation device has a pre-extraction internal space containing the sample, and includes a separation agent or a separation membrane for separating a specific component in the sample from the sample in the pre-extraction internal space, and an extraction outlet provided below the separation agent or the separation membrane, through which the sample in the pre-extraction internal space is extracted,
wherein the controlling method comprises:
determining the preprocessing procedure to be executed to each sample based on analysis items for each sample previously designated by an analyst;
controlling preprocessing performed in each of the plurality of stirring ports and the plurality of filtration ports;
controlling availability of each of the plurality of stirring ports and the plurality of filtration ports by remembering the plurality of stirring ports and the plurality of filtration ports that have received the separation device or collecting container of the preprocessing container or by using sensors provided to each of the plurality of stirring ports and the plurality of filtration ports that senses presence of the separation device or collecting container of the preprocessing container within the respective stirring ports and the respective filtration ports;
wherein the preprocessing procedure to be executed to each sample is including the filtration processing and the stirring processing,
wherein the controlling method further comprises:
checking availability of the plurality of stirring ports,
setting the separation device containing the sample in one of the plurality of stirring ports based on a determination that the one of the stirring ports is available;
executing the stirring processing to the separation device set in the one of the stirring ports;
while the stirring processing to the separation device is executed, checking availability of the plurality of filtration ports and setting the collecting container in one of the plurality of filtration ports based on a determination that the one of the filtration ports is available;
after the stirring processing to the separation device is completed, setting the separation device, to which the stirring processing has been executed, on the collecting container set in the one of the filtration ports, and executing the filtration processing by applying negative pressure to the sample in the separation device set on the collecting container so that the sample is extracted from the separation device to the collecting container.

### Claim 2 (depends on 1)

The controlling method according to claim 1, the preprocessing apparatus further comprising:
a pressure applying device used for applying a pressure to the sample in the preprocessing container set in a filtration port of the plurality of filtration ports so that the sample in the separation device set in the filtration port is extracted to form the extracted sample to be provided from the extraction outlet to the collecting container through the separation agent or the separation membrane.

### Claim 3 (depends on 2)

The controlling method according to claim 2, wherein
each of the separation device and the collecting container includes a flange part which is a circumferential expansion of an outer circumferential surface, and
the carrying mechanism is configured to carry the separation device and the collecting container with the holding part being engaged with the flange part of each of the separation device and the collecting container and with the separation device and the collecting container being carried separately by the holding part.

### Claim 4 (depends on 2)

The controlling method according to claim 2, wherein
each stirring mechanism is used for causing the separation device set in each of the plurality of stirring ports to periodically move in a horizontal plane, to stir the sample in the separation device.

### Claim 5 (depends on 4)

The controlling method according to claim 4, wherein each stirring mechanism is provided individually for each stirring port of the plurality of stirring ports, respectively.

### Claim 6 (depends on 2)

The controlling method according to claim 2, the preprocessing apparatus further comprises
a sample transfer part which includes a transfer port for receiving the collecting container containing the extracted sample which is provided from the separation device, and a port moving mechanism for moving the transfer port towards an outside of the preprocessing apparatus to an end portion of the sample transfer part, the sample transfer part placing the collecting container at the end portion of the sample transfer part by moving the transfer port, in which the collecting container is set, towards the outside of the preprocessing apparatus.

### Claim 7 (depends on 2)

The controlling method according to claim 2, the preprocessing apparatus further comprises
a drying/solidifying mechanism, which is configured to blow a drying/solidifying gas, serving as a third type of the preprocessing devices for individually supplying a drying/solidifying gas to the collecting container set in the filtration port, wherein
the third type of the preprocessing devices is configured to perform, after extraction processing is performed on the sample to form the extracted sample which is required to be dried and solidified, drying/solidifying processing in which the drying/solidifying gas is blown to the extracted sample in the collecting container.

### Claim 8 (depends on 7)

The controlling method according to claim 7, wherein
the drying/solidifying mechanism comprises a plurality of drying/solidifying-gas supply nozzles so as to have a drying/solidifying-gas supply nozzle of the plurality of drying/solidifying-gas supply nozzles provided to each of the filtration ports, respectively, each of the plurality of drying/solidifying-gas supply nozzles spouting a drying/solidifying gas from a tip, wherein
the third type of the preprocessing devices is configured to perform drying/solidifying processing in which the carrying mechanism places the drying/solidifying-gas supply nozzle in an opening in an upper surface of the collecting container in the corresponding filtration port, and a drying/solidifying gas is blown to the extracted sample in the collecting container from the tip of the drying/solidifying-gas supply nozzle.

### Claim 9 (depends on 1)

The controlling method according to claim 1, the preprocessing apparatus further comprises:
a dispensation port, provided in a position on the path of the holding part, configured to receive the separation device from the holding part of the carrying mechanism;
a sample setting part configured to hold a plurality of sample containers each containing a bulk sample of one or more bulk samples and place a desired sample container in a predetermined sampling position by moving the plurality of sample containers in a horizontal plane; and
a sampling part configured to suck a portion of a bulk sample as the sample from one of the plurality of the sample containers is placed in the sampling position, and dispenses the sample to the separation device set in the dispensation port.

### Claim 10 (depends on 9)

The controlling method according to claim 9, wherein
the sampling part includes a sampling arm configured to hold a nozzle, sucking and dispensing the sample, on an end of the sampling arm,
the sampling arm is connected to a vertical shaft at a base end of the sampling arm, and
the sampling arm rotates about the vertical shaft in a horizontal plane to move the nozzle in such a manner that the nozzle makes an arc-shaped path.

### Claim 11 (depends on 9)

The controlling method according to claim 9, the preprocessing apparatus further comprises:
a reagent setting part configured to hold a plurality of reagent containers each containing a bulk reagent to be added to each of the respective one or more samples;
a reagent arm that is configured to place a desired reagent container in a predetermined reagent taking position by moving the reagent containers in a horizontal plane; and
a reagent addition part configured to suck a portion of a reagent as the reagent from a reagent container placed in the predetermined reagent taking position, and dispenses the reagent to the separation device set in the dispensation port.

### Claim 12 (depends on 11)

The controlling method according to claim 11, wherein
the reagent addition part includes the reagent arm configured to hold a nozzle, sucking and dispensing the reagent, on an end of the reagent arm,
the reagent arm is connected to a vertical shaft at a base end of the reagent arm, and
the reagent arm rotates about the vertical shaft in a horizontal plane to move the nozzle in such a manner that the nozzle makes an arc-shaped path.

### Claim 13 (depends on 1)

The controlling method according to claim 1, wherein
the carrying mechanism includes a carrying arm which includes the holding part on an end of the carrying arm,
the carrying arm is connected to a vertical shaft at a base end of the carrying arm, and
the carrying arm rotates about the vertical shaft in a horizontal plane to move the holding part in such a manner that the holding part makes an arc-shaped path.