# US 11,441,178 B2 · Surface stabilization of biosensors

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- Number: US 11,441,178 B2
- Document ID: US11441178B2
- Status: Granted
- Kind: B2
- Application number: 16/824,920
- Filed: 2020-03-20
- Published: 2020-07-09
- Granted: 2022-09-13
- Earliest claimed: 2015-03-05
- Assignees: Life Technologies Corporation
- Applicants: Life Technologies Corporation
- Inventors: Phil Waggoner; James A. Ball; Wolfgang Hinz; Michael L. Minto; Scott Parker; David M. Cox; Alexander Mastroianni; Jeremy Gray; Marc Glazer; Kimberly Gorrell
- Web page: https://patentgrep.com/patent/US11441178B2

## CPC

- C12Q 1/6869: Methods for sequencing
- G01N 27/4145: {specially adapted for biomolecules, e.g. gate electrode with immobilised receptors}
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

A sensor apparatus includes a substrate, a semiconductor device disposed over the substrate, the semiconductor device having a surface electrode structure, and a saccharide coating formed over the surface electrode structure. The saccharide coating can be removed prior to use. The semiconductor device can further include a well and optionally a bead disposed in the well.

## Claims

### Claim 1

A method of preparing a sensor apparatus, the method comprising:
applying a cleaning solution through a flow cell over a semiconductor device the semiconductor device including an array of field effect transistor (FET) sensors, and an array of wells formed over the array of FET sensors; the array of wells and array of FET sensors forming a sensor chip with a sensor chip surface;
applying a saccharide solution through the flow cell, the solution including a saccharide; and
removing the saccharide solution from the flow cell, a layer of the saccharide remaining over the sensor chip surface.

### Claim 2 (depends on 1)

The method of claim 1, wherein applying the cleaning solution includes flowing a basic solution through the flow cell prior to applying the saccharide solution.

### Claim 3 (depends on 1)

The method of claim 1, wherein applying the cleaning solution includes flowing a non-aqueous surfactant solution through the flow cell prior to applying the saccharide solution.

### Claim 4 (depends on 1)

The method of claim 1, wherein the saccharide includes a monosaccharide, a disaccharide, a polysaccharide, a derivation thereof, or a combination thereof.

### Claim 5 (depends on 1)

The method of claim 1, wherein the saccharide solution includes the saccharide in a range of 5 wt % to 30 wt %.

### Claim 6 (depends on 1)

The method of claim 1, wherein the saccharide solution includes a surfactant in a range of 0.01 wt % to 10.0 wt %.

### Claim 7 (depends on 2)

The method of claim 2, wherein flowing a basic solution through the flow cell comprises flowing a sodium hydroxide solution through the flow cell.

### Claim 8 (depends on 3)

The method of claim 3, wherein the non-aqueous surfactant solution includes a sulfonic acid surfactant in a non-aqueous solvent.

### Claim 9 (depends on 8)

The method of claim 8, wherein the sulfonic acid surfactant includes dodecyl sulfonic acid, dioctyl sulfosuccinic acid, or dodecyl benzene sulfonic acid, and salts thereof.

### Claim 10 (depends on 8)

The method of claim 8, wherein the non-aqueous surfactant solution is 5 wt % dodecyl benzene sulfonic acid in undecane.

### Claim 11 (depends on 4)

The method of claim 4, wherein the monosaccharide includes glucose, fructose, or galactose.

### Claim 12 (depends on 4)

The method of claim 4, wherein the disaccharide includes sucrose, trehalose, maltose, or lactose.

### Claim 13 (depends on 6)

The method of claim 6, wherein the surfactant includes a non-ionic fluorosurfactant, an alkyl polysaccharide surfactant, an alkyl sulfonate surfactant or a zwitterionic surfactant.

### Claim 14 (depends on 6)

The method of claim 6, wherein the saccharide solution including the surfactant is a sucrose solution in a range of 10 wt % to 20 wt % sucrose with an alkyl polysaccharide surfactant in a range of 0.05 wt % to 0.1 wt %.

### Claim 15 (depends on 6)

The method of claim 6, wherein the saccharide solution including the surfactant is a sucrose solution in a range of 10 wt % to 20 wt % sucrose with a zwitterionic surfactant in a range of 0.1 wt % to 0.2 wt %.

### Claim 16 (depends on 1)

The method of claim 1, wherein the array of FET sensors is an array of chemical FET (chemFET) sensors.

### Claim 17 (depends on 16)

The method of claim 16, wherein the array of chemFET sensors is an array of ion selective FET (ISFET) sensors.