Method for processing polynucleotide-containing samples

A system comprising:

• a tubular vessel comprising an opened end and an opening, the opening opposite the opened end of the tubular vessel;
• a valve for opening and closing the opening;
• a closure configured to close the opened end;
• a gas pressure generator disposed within the tubular vessel;
• and
• a region disposed within the tubular vessel and positioned between the gas pressure generator and the opening,
• wherein the tubular vessel is configured to receive a polynucleotide-containing sample through the opened end and into the region, wherein the polynucleotide-containing sample is configured to be heated within the region once the opened end is closed with the closure, and
• wherein the gas pressure generator is configured to expel a solution with released polynucleotides from the tubular vessel through the opening.