# US 11,441,168 B2 · Method for assaying the activity of spermidine/spermine N1-acetyltransferase

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- Number: US 11,441,168 B2
- Document ID: US11441168B2
- Status: Granted
- Kind: B2
- Application number: 17/406,956
- Filed: 2021-08-19
- Published: 2022-02-03
- Granted: 2022-09-13
- Earliest claimed: 2011-11-16
- Assignees: BioMark Technologies Inc.
- Applicants: BioMark Technologies Inc.
- Inventors: Brian Cheng; Rashid Bux; Daniel Sitar
- Web page: https://patentgrep.com/patent/US11441168B2

## CPC

- C12Q 1/48: involving transferase
- G01N 33/57557: {of other specific parts of the body, e.g. brain}
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

A method for assaying activity of the enzyme spermidine/spermine N1-acetyltransferase (SSAT) uses SSAT substrates by detecting their acetylated forms. SSAT substrates may include rimantadine and tocainide wherein their metabolism occurs in part by the action of the inducible enzyme SSAT to produce the acetylated metabolites N-acetylrimantadine and N-acetyltocainide respectively. SSAT activity may be correlated to pathologic conditions.

## Claims

### Claim 1

A method for determining activity of spermine/spermidine N1-acetyltransferase (SSAT) in a mammal, the method comprising:
(a) providing a biological sample from the mammal obtained 2-24 hours following administration of a single dose of rimantadine; and;
(b) quantifying the level of N-acetyl rimantadine in the biological sample, thereby determining SSAT activity in the mammal.

### Claim 2 (depends on 1)

The method of claim 1, wherein the biological sample is obtained from the mammal 2-4 hours following administration of the single dose of rimantadine.

### Claim 3 (depends on 1)

The method of claim 1, wherein the biological sample is blood.

### Claim 4 (depends on 1)

The method of claim 1, wherein the biological sample is urine.

### Claim 5 (depends on 1)

The method of claim 1, wherein the biological sample is saliva.

### Claim 6

A method for determining activity of spermine/spermidine N1-acetyltransferase (SSAT) in a mammal, the method comprising:
(a) administering a single dose of rimantadine to the mammal;
(b) collecting a biological sample from the mammal 2-24 hours following the single dose rimantadine administration; and
(c) quantifying the level of N-acetyl rimantadine in the biological sample, thereby determining SSAT activity in the mammal.

### Claim 7 (depends on 6)

The method of claim 6, wherein the biological sample is collected from the mammal 2-4 hours following administration of the single dose of rimantadine.

### Claim 8 (depends on 6)

The method of claim 6, wherein the biological sample is blood.

### Claim 9 (depends on 6)

The method of claim 6, wherein the biological sample is urine.

### Claim 10 (depends on 6)

The method of claim 6, wherein the biological sample is saliva.