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US 11,441,162 B2Granted
Human gut microbiome-derived biosynthetic enzymes for production of fatty acid amides
AssigneeMassachusetts Institute of Technology
InventorsChristopher A. Voigt, Fang-Yuan Chang
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Abstract
Disclosed herein, in some embodiments, are vectors encoding biosynthetic enzymes from gut microbiome-derived bacterium (e.g., Clostridia enzymes), engineered cells comprising the vectors, and methods of using biosynthetic enzymes from gut microbiome-derived bacterium (e.g., Clostridia enzymes) to produce fatty acid amides.
Claims31
A method of producing fatty acid amides, the method comprising:
- contacting a composition, comprising one or more exogenous fatty acids and one or more amines, with a set of biosynthetic enzymes which are human gut microbiome-derived clostridia biosynthetic enzymes in an effective amount to produce a fatty acid amide, wherein the set of biosynthetic enzymes are recombinant proteins expressed from one or more vectors comprising a single operon, wherein the operon comprises genes encoding at least a fatty acyl transferase, an acyl carrier protein (ACP), and a fatty acyl-ACP synthetase.
A method of producing a fatty acid amide, the method comprising
- (i) administering to a host a plurality of vectors comprising a first vector, a second vector, and a third vector,
- wherein each of the plurality of vectors comprises a human gut microbiome-derived bacterium gene under the control of a regulatory element;
- and
- wherein the gene on the first vector, the second vector and the third vector encode a fatty acyl transferase, an acyl carrier protein, and fatty acyl-ACP synthetase, respectively;
- and
- (ii) contacting the host with a composition in an effective amount to produce a fatty acid amide in the host.
3 more elements
A method of producing a fatty acid amide, the method comprising
- (i) administering to a host a vector comprising genes, wherein each of the genes is under the control of a regulatory element, wherein each of the genes is derived from a human gut microbiome-derived bacterium, and wherein the genes encode a fatty acyl transferase, an acyl carrier protein, and fatty acyl-ACP synthetase;
- and
- (ii) contacting the host with a composition in an effective amount to produce a fatty acid amide in the host.
Dependencies are inferred from the claim text.