# US 11,441,161 B2 · Removing polyphenol contaminants from feedstock-based polyphenols > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 11,441,161 B2 - Document ID: US11441161B2 - Status: Granted - Kind: B2 - Application number: 16/631,190 - Filed: 2018-07-26 - Published: 2020-07-09 - Granted: 2022-09-13 - Earliest claimed: 2017-07-26 - Assignees: Immortazyme Company - Applicants: Vincent Yacyshyn - Inventors: Vincent Yacyshyn - Web page: https://patentgrep.com/patent/US11441161B2 ## CPC - C12P 7/22: aromatic - C12P 7/625: Polyesters of hydroxy carboxylic acids - C12P 7/10: substrate containing cellulosic material - C12P 7/64: Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats - C12P 19/02: Monosaccharides (2-ketogulonic acid C12P7/60) - Y02E 50/10: Biofuels, e.g. bio-diesel - C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)} - Y02E: REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION ## Abstract Provided is a method of producing a mixture of pure feedstock-based native polyphenols from a feedstock. Contaminant polyphenols are first removed from an enzyme solution for converting feedstock to a product to produce a polyphenol reduced enzyme solution. The polyphenol reduced enzyme solution is combined with the feedstock and the feedstock is converted to a product and by-product. Heretofore, there has been no process available to reduce or remove the contaminant phenols introduced to the feedstock by commercial enzyme solutions. This method allows for the removal of contaminant phenols prior to introduction to the processing stream and subsequent harvesting of pure feedstock 6 based native polyphenols. The pure feedstock-based polyphenols are removed from the product or by-product to produce a pure mixture of feedstock-based polyphenols. ## Claims ### Claim 1 A method of producing a pure mixture of native feedstock-based polyphenols from a feedstock for making alcohols or sugars, comprising the steps of: prior to enzymatic processing of the feedstock removing contaminating polyphenols from an enzyme solution for converting feedstock to an alcohol or sugar to produce a polyphenol removed enzyme solution; combining the polyphenol removed enzyme solution with the feedstock; enzymatic processing of the feedstock without contaminating phenols being present by converting the feedstock to at least one of alcohol or sugar, wherein contaminating polyphenols are phenols or polyphenols not sourced from the feedstock; and producing the pure mixture of native feedstock-based polyphenols from the feedstock free of the contaminating polyphenols from the enzyme solution by separating native feedstock-based polyphenols from at least one of the alcohol, the sugar, or a by-product of the conversion of the feedstock to the sugar or the alcohol, wherein a native composition of the feedstock-based polyphenols is preserved in the pure mixture of native feedstock-based polyphenols, and the pure mixture of native feedstock-based polyphenols free of a contaminating polyphenols is suitable for human consumption to provide a reduction of at least one of a vasoreactivity, a cholesterol level, a dementia, and a cardiovascular disease. ### Claim 2 (depends on 1) The method of claim 1, wherein the feedstock comprises a grain. ### Claim 3 (depends on 1) The method of claim 1, wherein the by-product comprises an oil. ### Claim 4 A method of producing a pure mixture of native feedstock-based polyphenols from a feedstock comprising a renewable biological material, comprising the steps of: prior to enzymatic processing of the feedstock, removing contaminating polyphenols from an enzyme solution for converting the feedstock to a product to produce a polyphenol removed enzyme solution; combining the polyphenol removed enzyme solution with the feedstock; enzymatic processing of the feedstock without contaminating phenols being present by converting the feedstock to the product, wherein contaminating polyphenols are phenols or polyphenols not sourced from the feedstock; and producing the pure mixture of native feedstock-based polyphenols free of the contaminating polyphenols from the enzyme solution by separating native feedstock-based polyphenols from the product or a by-product of the conversion of the feedstock to the product, wherein a native composition of the feedstock-based polyphenols is preserved in the pure mixture of native feedstock-based polyphenols, and the pure mixture of native feedstock-based polyphenols free of a contaminating polyphenols is suitable for human consumption to provide a reduction of at least one of a vasoreactivity, a cholesterol level, a dementia, and a cardiovascular disease. ### Claim 5 (depends on 4) The method of claim 4, wherein the product comprises a biofuel, a food product or an energy product. ### Claim 6 (depends on 4) The method of claim 4, wherein the feedstock comprises a grain. ### Claim 7 (depends on 4) The method of claim 4, wherein the by-product comprises an oil. ### Claim 8 (depends on 1) The method of claim 1, wherein the cardiovascular disease includes at least one of stroke, myocardial infarction, and cardiovascular death. ### Claim 9 (depends on 4) The method of claim 4, wherein the cardiovascular disease includes at least one of stroke, myocardial infarction, and cardiovascular death.