# US 11,441,145 B2 · Affinity matrix and devices for isolation and purification of RNA and DNA for point of care molecular devices > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 11,441,145 B2 - Document ID: US11441145B2 - Status: Granted - Kind: B2 - Application number: 16/810,798 - Filed: 2020-03-05 - Published: 2020-06-25 - Granted: 2022-09-13 - Earliest claimed: 2014-04-29 - Assignees: Accudx Corporation - Applicants: AccuDx Corporation - Inventors: Raveendran Pottathil - Web page: https://patentgrep.com/patent/US11441145B2 ## CPC - C12N 15/101: {by chromatography, e.g. electrophoresis, ion-exchange, reverse phase} - B01D 15/1871: {placed in series} - B01D 15/3804: {Affinity chromatography} - B01L 3/5023: {with a sample being transported to, and subsequently stored in an absorbent for analysis} - B01L 9/52: {Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips} - B01L 2300/043: Hinged closures - B01L 2300/048: enabling gas exchange, e.g. vents - B01L 2300/069: Absorbents; Gels to retain a fluid - B01L 2300/0825: Test strips - B01L 2400/0406: capillary forces - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) - B01D: SEPARATION (separating solids from solids by wet methods B03B, B03D; by pneumatic jigs or tables B03B; by other dry methods B07; magnetic or electrostatic separation of solid materials from solid materials or fluids, separation by high-voltage electric fields B03C; centrifuges, vortex apparatus B04; presses per se for squeezing-out liquid from liquid-containing material B30B9/02; treatment of water C02F, e.g. softening by ion-exchange C02F1/42; {arrangements of air intake cleaners in gas turbine plants F02C7/05} ; arrangements or mounting of filters in air-conditioning, air-humidification or ventilation F24F13/28) - B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE ## Abstract The present disclosure relates to nucleic acid extraction and purification methods and devices to accomplish the same. ## Claims ### Claim 1 A device for isolating nucleic acid from a biological sample comprising at least one nucleic acid, the device comprising: an affinity matrix comprising at least one nucleic acid binding moiety covalently bound to the affinity matrix, the at least one nucleic acid binding moiety selected from streptomycin and tetracycline, the affinity matrix free of nucleic acid prior to contacting the sample, the affinity matrix configured to non-specifically bind the at least one nucleic acid from the sample in response to contacting the sample, thereby binding the at least one nucleic acid to the affinity matrix; and an elution pad configured to retain an elution buffer, the elution buffer configured to elute the at least one nucleic acid from the nucleic acid-bound affinity matrix in response to the elution pad contacting the affinity matrix, thereby isolating the at least one nucleic acid. ### Claim 2 (depends on 1) The device of claim 1, further comprising a sample receiving pad configured to receive the sample comprising the at least one nucleic acid, the sample receiving pad further configured to retain a cell disruption reagent configured to disrupt at least some cells in the sample received by the sample receiving pad, the sample receiving pad further configured to receive a wash buffer, an edge of the sample receiving pad adjoining a first edge of the affinity matrix, the sample receiving pad configured to transport the at least some cells disrupted by the cell disruption reagent via capillary action to the affinity matrix in response to receiving the wash buffer. ### Claim 3 (depends on 2) The device of claim 2, further comprising a sink, an edge of the sink adjoining a second edge of the affinity matrix, the second edge of the affinity matrix opposite to the first edge of the affinity matrix, the sink configured to receive portions of the sample from the affinity matrix not eluted to the elution pad. ### Claim 4 (depends on 3) The device of claim 3, wherein the sink comprises a well. ### Claim 5 (depends on 3) The device of claim 3, wherein the sink comprises a sink pad. ### Claim 6 (depends on 3) The device of claim 3, further comprising a strip, wherein the sample receiving pad, the affinity matrix, and/or the sink are arrayed linearly on the strip. ### Claim 7 (depends on 6) The device of claim 6, further comprising a housing, the housing configured to house the strip, the sample receiving pad, the affinity matrix, the elution pad, and/or the sink. ### Claim 8 (depends on 7) The device of claim 7, wherein a bottom surface of the strip contacts a bottom interior surface of the housing, and where the sample receiving pad, the affinity matrix, and/or the sink are arrayed linearly on the top surface of the strip. ### Claim 9 (depends on 8) The device of claim 8, wherein the strip, the sample receiving pad, the affinity matrix, and/or the sink is removable from the housing. ### Claim 10 (depends on 8) The device of claim 8, further comprising an opening in a top surface of the housing above the receiving pad, thereby providing access to the receiving pad for applying the sample and/or the wash buffer to the receiving pad without opening the housing. ### Claim 11 (depends on 10) The device of claim 10, further comprising a hinged portion of the top surface of the housing, wherein the elution pad is attached to the hinged portion of the top surface of the housing such that the elution pad contacts the affinity matrix when the hinged portion of the top surface of the housing is in a closed position, thereby contacting the elution pad to the affinity matrix when the hinged portion of the top surface of the housing is in the closed position. ### Claim 12 (depends on 11) The device of claim 11, wherein the hinged portion of the top surface of the housing and the elution pad are removable from the housing. ### Claim 13 (depends on 1) The device of claim 1, wherein the at least one nucleic acid binding moiety is streptomycin. ### Claim 14 (depends on 1) The device of claim 1, wherein the at least one nucleic acid binding moiety is tetracycline.