# US 11,441,143 B2 · Enzyme-carrier complex

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- Number: US 11,441,143 B2
- Document ID: US11441143B2
- Status: Granted
- Kind: B2
- Application number: 17/119,473
- Filed: 2020-12-11
- Published: 2021-04-22
- Granted: 2022-09-13
- Earliest claimed: 2019-01-31
- Assignees: Korea Univercity Research and Business Foundation
- Applicants: Korea University Research and Business Foundation
- Inventors: Jungbae Kim; Tae Hee Kim
- Web page: https://patentgrep.com/patent/US11441143B2

## CPC

- C12N 11/14: Enzymes or microbial cells immobilised on or in an inorganic carrier
- C12N 9/96: Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
- C12N 11/08: the carrier being a synthetic polymer
- C12Y 305/01004: Amidase (3.5.1.4)
- Y02E 60/50: Fuel cells
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C12Y: ENZYMES
- Y02E: REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION

## Abstract

The present invention relates to an enzyme-carrier complex, and more particularly to the adsorption and stabilization of an enzyme on the surface of a carrier, and an enzyme-carrier complex with secured enzyme stability so that an enzyme immobilized by a hydrophobic interaction exhibits long-term enzymatic activity.

## Claims

### Claim 1

An enzyme-carrier complex comprising: a hydrophobic carrier which is a carbon nanotube; and one or more acylases adsorbed on a surface of the hydrophobic carrier,
wherein the enzyme-carrier complex does not form aggregates in a dispersed state in water.

### Claim 2 (depends on 1)

The enzyme-carrier complex of claim 1, wherein the hydrophobic carrier further comprises a first functional group on a surface thereof to induce a hydrophobic interaction with the enzyme.

### Claim 3 (depends on 2)

The enzyme-carrier complex of claim 2, wherein the one or more acylases further comprises a second functional group for inducing a hydrophobic interaction with the first functional group.

### Claim 4 (depends on 3)

The enzyme-carrier complex of claim 3, wherein the second functional group for inducing a hydrophobic interaction comprises one or more functional groups selected from the group consisting of a halogenated alkyl group, an organosilicon group, an alkyl group, a vinyl group, an allyl group, and an aryl group.

### Claim 5 (depends on 1)

An electrode for a biofuel cell, the electrode comprising the enzyme-carrier complex according to claim 1.

### Claim 6 (depends on 1)

An electrode for a biosensor, the electrode comprising the enzyme-carrier complex according to claim 1.

### Claim 7 (depends on 1)

A carbon dioxide conversion system comprising the enzyme-carrier complex according to claim 1.

### Claim 8 (depends on 1)

An antifouling system comprising the enzyme-carrier complex according to claim 1.