# US 11,441,139 B2 · α-Amylase variants

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- Number: US 11,441,139 B2
- Document ID: US11441139B2
- Status: Granted
- Kind: B2
- Application number: 16/639,909
- Filed: 2018-08-20
- Published: 2021-06-24
- Granted: 2022-09-13
- Earliest claimed: 2017-08-18
- Assignees: Danisco US Inc (157111)
- Applicants: Danisco US Inc.
- Inventors: Jonathan K. Lassila; Sandra W. Ramer
- Web page: https://patentgrep.com/patent/US11441139B2

## CPC

- C12N 9/2414: {Alpha-amylase (3.2.1.1.)}
- C12N 9/2417: {from microbiological source}
- C11D 3/386: Preparations containing enzymes {, e.g. protease or amylase}
- C12P 19/14: produced by the action of a carbohydrase {(EC 3.2.x)}, e.g. by alpha-amylase {, e.g. by cellulase, hemicellulase}
- C12Y 302/01001: Alpha-amylase (3.2.1.1)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C11D: DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)}
- C12Y: ENZYMES

## Abstract

Disclosed are compositions and methods relating to variant alpha-amylases. The variant alpha-amylases are useful, for example, for starch liquefaction and saccharification, for cleaning starchy stains in laundry, dishwashing, and other applications, for textile processing (e.g., desizing), in animal feed for improving digestibility, and for baking and brewing.

## Claims

### Claim 1

A recombinant variant α-amylase comprising an amino acid sequence having at least 97% amino acid sequence identity to the amino acid sequence of SEQ ID NO: 2, excluding the wild-type Amy707 enzyme having the amino acid sequence of SEQ ID NO: 2.

### Claim 2 (depends on 1)

A composition for liquefying starch comprising the variant α-amylase of claim 1.

### Claim 3 (depends on 1)

A detergent composition comprising the variant α-amylase of claim 1.