Modified gene sequences encoding choline oxidase and a method for preparing betaine using the same

A modified gene sequence of codA gene comprising the nucleotide sequence of SEQ ID NO:

• 2 for encoding choline oxidase;
• wherein
• the choline oxidase encoded by said nucleotide sequence has stability in the pH range 7.0 to 9.0;
• the choline oxidase encoded by said nucleotide sequence has stability at a temperature range of 10-50 ° C.;
• the choline oxidase encoded by said nucleotide sequence has stability at a temperature range of 10-50 ° C. for up to 120 minutes;
• and
• the choline oxidase thus encoded is useful to produce betaine from choline.

A method for producing betaine from choline comprising the steps of:

• a) making a reaction mixture comprising of choline aqueous solution, an enzyme and optionally a hydrogen peroxide catalyzing agent;
• b) carrying out a reaction using the reaction mixture in a reactor;
• and
• c) on completion of the reaction, separating the enzyme from the mixture;
• wherein the enzyme comprises choline oxidase encoded by the nucleotide sequence of SEQ ID NO:
• 2;
• and
• the method has choline to betaine conversion efficiency of 1:1 molar ratio.