# US 11,440,013 B2 · System for preparing a sample > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 11,440,013 B2 - Document ID: US11440013B2 - Status: Granted - Kind: B2 - Application number: 16/337,443 - Filed: 2017-09-29 - Published: 2020-01-30 - Granted: 2022-09-13 - Earliest claimed: 2016-09-30 - Assignees: Koninklijke Philips N.V. - Applicants: Koninklijke Philips N.V. - Inventors: Matthias Irmscher; Toon Hendrik Evers; Marlieke Joan Overdijk; Thomas Johannes Van Gijsel; Stefan Johannes Hendrikus Toonen - Web page: https://patentgrep.com/patent/US11440013B2 ## CPC - B01L 3/5029: {using swabs} - B01L 3/523: {with means for closing or opening} - F04B 7/04: in which the valving is performed by pistons and cylinders coacting to open and close intake or outlet ports - F04B 19/006: {Micropumps (F04B43/043 and F04B43/095 take precedence)} - G01N 1/286: {involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising (microtomes G01N1/06; pulverising in general B02C; mixing in general B01F)} - B01L 2200/0647: Handling flowable solids, e.g. microscopic beads, cells, particles - B01L 2200/0689: Sealing - B01L 2200/10: Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept - B01L 2200/16: Reagents, handling or storing thereof - B01L 2300/0672: Integrated piercing tool - B01L 2300/0681: Filter - B01L 2300/087: Multiple sequential chambers - B01L 2400/0478: pistons - B01L 2400/0481: squeezing of channels or chambers - B01L 2400/049: vacuum - B01L 2400/065: sliding valves - G01N 2001/2873: {Cutting or cleaving} - B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE - F04B: POSITIVE-DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS (machines for liquids, or pumps, of rotary-piston or oscillating-piston type F04C; non-positive-displacement pumps F04D; pumping of fluid by direct contact of another fluid or by using inertia of fluid to be pumped F04F) - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) ## Abstract A device is provided comprising a receiving chamber for receiving a patient's sample, a first reagent reservoir and a second reagent reservoir. The receiving chamber may be configured for receiving at least an end portion of a sample collecting element, with the patient's sample being collected by that sample collecting element. The first reagent reservoir may generally be configured to accommodate a liquid reagent which may include a lysing agent, i.e. the liquid may be a kind of a lysis buffer. Likewise, the second reagent reservoir may generally be configured to accommodate a dry reagent which may include magnetic particles. The device may further comprise at least one of the following two actuators, a first actuator for supplying a first reagent from the first reagent reservoir to the sample and a second actuator for supplying a second reagent from the second reagent reservoir to the resulting mixture. ## Claims ### Claim 1 A device for preparing a patient's sample before analysis, comprising: a housing having a central axis; and an actuator; wherein the housing comprises a receiving chamber and a reagent reservoir, wherein the receiving chamber comprises an opening; wherein the receiving chamber is configured for receiving at least a portion of the patient's sample through the opening, wherein the reagent reservoir is configured for receiving a reagent, wherein the actuator is rotatable relative to housing about the central axis of the housing, wherein rotation of the actuator: closes the opening of the receiving chamber, and causes contact between the reagent and the patient's sample, such that the patient's sample is at least partially mixed with the reagent wherein the actuator is configured to cut a portion of a sample collecting member, wherein the sample collecting member protrudes out of the receiving chamber as the actuator rotates about the central axis of the housing. ### Claim 2 (depends on 1) The device of claim 1, wherein the reagent comprises a lysing agent. ### Claim 3 (depends on 1) The device of claim 1, further comprising a blocking member, wherein the blocking member is configured for blocking a reverse rotation of the actuator after the receiving chamber is closed by the actuator. ### Claim 4 (depends on 3) The device of claim 3, wherein the actuator is further configured for sealing the receiving chamber when closing the opening. ### Claim 5 (depends on 1) The device of claim 1, wherein the actuator is configured to cut a first portion of the sample collecting member, wherein the first portion of the sample collecting member protrudes out of the receiving chamber, wherein the actuator is configured to close and seal the receiving chamber, wherein the actuator is configured to release the reagent from the reagent reservoir to a second portion of the sample collecting member, wherein the second portion of the sample collecting member is in the receiving chamber. ### Claim 6 (depends on 5) The device of claim 5, wherein a closing member comprises two gripping members, wherein the two gripping members are moving towards each other when the actuator is moved to close the opening. ### Claim 7 (depends on 1) The device of claim 1, wherein the receiving chamber comprises a closure, wherein the closure comprises a cutting portion; wherein the cutting portion is configured to cut a portion of a sample collecting member, wherein the portion of the sample collecting member protrudes out of the receiving chamber; and wherein a supplying actuator also acts as the actuator, wherein the supplying actuator comprises a the closing member, wherein the closing member is arranged to close the opening of the receiving chamber, and wherein the supplying actuator comprises a cutting actuator, wherein the cutting actuator activates the cutting of the protruding portion of a sample collecting member. ### Claim 8 (depends on 7) The device of claim 7, wherein the supplying actuator is configured to move along a circumference of the receiving chamber. ### Claim 9 (depends on 7) The device of claim 7, wherein the supplying actuator comprises a wall portion, wherein the wall portion causes the reagent to be supplied from the reagent reservoir when the actuator moves relative to the receiving chamber. ### Claim 10 (depends on 7) The device of claim 7, further comprising a second reagent reservoir, wherein the second reagent reservoir is configured to receive a second reagent, wherein a second actuator is configured to supply the liquid to the second reagent reservoir. ### Claim 11 A device for preparing a patient's sample before analysis, comprising: a housing having a central axis; and an actuator; wherein the housing comprises a receiving chamber and a reagent reservoir, wherein the receiving chamber comprises an opening; wherein the receiving chamber is configured for receiving at least a portion of the patient's sample through the opening, wherein the reagent reservoir is configured for receiving a reagent, wherein the actuator is rotatable relative to housing about the central axis of the housing, wherein rotation of the actuator: closes the opening of the receiving chamber, and causes contact between the reagent and the patient's sample, such that the patient's sample is at least partially mixed with the reagent, wherein the receiving chamber comprises a closure, wherein the closure comprises a cutting portion, wherein the cutting portion is configured to cut a portion of a sample collecting member, wherein the portion of the sample collecting member protrudes out of the receiving chamber; and wherein a supplying actuator also acts as the actuator, wherein the supplying actuator comprises a closing member, wherein the closing member is arranged to close the opening of the receiving chamber, wherein the supplying actuator comprises a cutting actuator, wherein the cutting actuator activates the cutting of the protruding portion of a sample collecting member. ### Claim 12 (depends on 11) The device of claim 11, wherein the supplying actuator is configured to move along a circumference of the receiving chamber. ### Claim 13 (depends on 11) The device of claim 11, wherein the supplying actuator comprises a wall portion, wherein the wall portion causes the reagent to be supplied from the reagent reservoir when the actuator moves relative to the receiving chamber. ### Claim 14 (depends on 11) The device of claim 11, further comprising a second reagent reservoir, wherein the second reagent reservoir is configured to receive a second reagent, wherein a second actuator is configured to supply the liquid to the second reagent reservoir.