# US 11,439,680 B2 · Veterinary composition of marine algae and Andrographis sp extracts, which can be used to treat infections in fish

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- Number: US 11,439,680 B2
- Document ID: US11439680B2
- Status: Granted
- Kind: B2
- Application number: 15/565,091
- Filed: 2016-04-06
- Published: 2018-10-11
- Granted: 2022-09-13
- Earliest claimed: 2015-04-08
- Applicants: Maqui New Life S.A.
- Inventors: Paula Miranda Campos; Claudio Rabuco Jeraldino
- Web page: https://patentgrep.com/patent/US11439680B2

## CPC

- A61K 36/53: Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
- A23K 20/163: Sugars; Polysaccharides
- A23K 50/80: for aquatic animals, e.g. fish, crustaceans or molluscs
- A61K 36/03: Phaeophycota or phaeophyta (brown algae), e.g. Fucus
- A61K 36/19: Acanthaceae (Acanthus family)
- A61P 31/04: Antibacterial agents
- A61P 31/12: Antivirals
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A23K: FODDER
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS

## Abstract

Veterinary composition comprising an extract of seaweed containing at least 5% fucoidians and an Andrographis sp plant extract containing at least 5% of andrographolide, which is useful in the control and prevention of infections produced by intracellular microorganisms in fishes.

## Claims

### Claim 1

An immunostimulant composition administered to fish comprising:
an extract of Andrographis sp containing andrographolide and a seaweed extract containing fucoidan, wherein the ratio of andrographolide:fucoidan is in the ratio of 100:450;
wherein the seaweed extract comprises an aqueous extract of brown algae, wherein the brown algae is selected from the group consisting of Fucus vesiculosus, Fucus evanescens, Fucus distichus, Fucus serratus, Pelvetia wrightii, Ascophyllum nodosum, Himanthalia Lorea, Bifurcaria bifurcata, Sargassum stenophyllum, Hizikia fusiforme, Durvillaea antarctica, Lessonia nigrescens, Lessonia trabeculata, Lessonia vadosa, Macrocystis pyrifera, Undaria pinnatifida, Padina pavonica, Laminarfa angustata, Laminaria japonica, Ecklonia kurome, Adenocystis utricularis, Dictyota menstrualis, Spatoglossum schroederi and Chordaria; and
wherein the brown algae belongs to either (1) a fucal order of algae, each member of the fucal order of algae comprising one or more binding fucose units having glycosidic bonds of type (1-3) or (1-4) and sulfated groups located at positions C-2, C-3, or C-4, or (2) a Laminare order of algae, each member of the Laminare order of algae comprising one or more binding fucose units having glycosidic bonds of type (1-2) or (1-3) and sulfated groups located at positions C-2 or C-4.

### Claim 2 (depends on 1)

The composition of claim 1, wherein each member of the fucal order of algae and each member of the Laminare order of algae also comprises a galactan fraction in the bonds (1-3) and (1-6) of the sulphated groups at position C4.

### Claim 3 (depends on 1)

The composition of claim 1, wherein said composition is formulated as an immunostimulant incorporated in fish food for treating infections.

### Claim 4 (depends on 1)

The composition of claim 1, wherein said composition is a fish food additive having andrographolide and fucoidan.

### Claim 5 (depends on 1)

The composition of claim 1, wherein the extract of Andrographis sp is selected from the group consisting of Andrographis paniculata, Andrographis affinis Nees, Andrographis beddomei, Andrographis echioides Nees, Andrographis elongata, Andrographis humifusa, Andrographis lineata Nees, Andrographis macrobotrys Nees, Andrographis nallamalayana, Andrographis neesiana, Andrographis ovata, Andrographis paniculata nees, Andrographis rothii, Andrographis serpyllifolia, Andrographis viscosula Nees, Andrographis viscosula var. explicata and Andrographis wightiana.

### Claim 6 (depends on 1)

The composition of claim 1, wherein the seaweed extract is an extract of Macrocystes pyrifera.

### Claim 7 (depends on 1)

A method for controlling and preventing infections caused by intracellular microorganisms in fishes which comprises mixing the composition of claim 1 with the fishes' food or diet.

### Claim 8 (depends on 7)

The method of claim 7, comprising mixing the composition with the fishes' food or diet in a ratio in the range of 0.5 to 2.5 kg per ton of food.

### Claim 9 (depends on 8)

The method of claim 8, comprising mixing the composition with the fishes' food or diet at a rate of 1 kg per ton of food.