# US 11,439,677 B2 · Vibrio anguillarum bacteriophage VIB-ANP-1 and use thereof for inhibiting proliferation of Vibrio anguillarum bacteria

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- Number: US 11,439,677 B2
- Document ID: US11439677B2
- Status: Granted
- Kind: B2
- Application number: 16/329,931
- Filed: 2017-08-21
- Published: 2019-06-20
- Granted: 2022-09-13
- Earliest claimed: 2016-09-02
- Assignees: Intron Biotechnology, Inc.
- Applicants: Intron Biotechnology, Inc.
- Inventors: Seong Jun Yoon; Soo Youn Jun; An Sung Kwon; Soon Hye Hwang; Hyun Min Song; Ji In Jung; Sang Hyeon Kang
- Web page: https://patentgrep.com/patent/US11439677B2

## CPC

- A61K 35/76: Viruses; Subviral particles; Bacteriophages
- A23K 10/18: of live microorganisms
- A23K 20/153: Nucleic acids; Hydrolysis products or derivatives thereof
- A23K 50/80: for aquatic animals, e.g. fish, crustaceans or molluscs
- A61K 9/0014: {Skin, i.e. galenical aspects of topical compositions (non-active ingredients are additionally classified in A61K47/00; A61K9/0009, A61K9/0021, A61K9/7015, A61K9/7023 take precedence; cosmetic preparations A61K8/00, A61Q; preparations for wound dressings or bandages A61L26/00)}
- A61P 31/04: Antibacterial agents
- C12N 7/00: Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof (preparing medicinal viral antigen or antibody compositions, e.g. virus vaccines, A61K39/00)
- C12N 2795/10321: Viruses as such, e.g. new isolates, mutants or their genomic sequences
- C12N 2795/10331: Uses of virus other than therapeutic or vaccine, e.g. disinfectant
- C12N 2795/10332: Use of virus as therapeutic agent, other than vaccine, e.g. as cytolytic agent
- C12N 2795/10333: Use of viral protein as therapeutic agent other than vaccine, e.g. apoptosis inducing or anti-inflammatory
- C12N 2795/10371: Demonstrated in vivo effect
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A23K: FODDER
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

The present invention relates to Siphoviridae bacteriophage Vib-ANP-1(accession number KCTC 13075BP) having the ability to specifically kill Vibrio anguillarum bacteria and a genome represented by SEQ ID NO: 1 and isolated from nature, and a method for prevention or treatment of Vibrio anguillarum bacterial infection by using a composition containing the same bacteriophage as an effective ingredient.

## Claims

### Claim 1

A feed additive composition comprising maltodextrin and 1×104 pfu/ml to 1×1015 pfu/ml or 1×104 pfu/g to 1×1015 pfu/g of Siphoviridae bacteriophage Vib-ANP-1 that has a specific antibacterial activity against Vibrio anguillarum and a 213.97 kb size of genome expressed by a nucleotide sequence of SEQ ID NO:1, and is deposited as accession number of KCTC 13075BP.

### Claim 2 (depends on 1)

The feed additive composition of claim 1, wherein the composition suppresses diseases caused by Vibrio anguillarum, or alleviates the pathological condition of the diseases caused by Vibrio anguillarum.

### Claim 3

A method for suppressing diseases caused by Vibrio anguillarum, or alleviating the pathological condition of the diseases caused by Vibrio anguillarum, wherein the method comprises a step of administering to an animal other than a human a composition comprising maltodextrin and 1×104 pfu/ml to 1×1015 pfu/ml or 1×104 pfu/g to 1×1015 pfu/g of Siphoviridae bacteriophage Vib-ANP-1 (Accession number: KCTC 13075BP), that has a specific antibacterial activity against Vibrio anguillarum and a 213.97 kb size of genome encoded by the nucleotide sequence of SEQ ID NO:1.