# US 11,439,604 B1 · Plant phenols extraction and activation thereof, to use as feed additive in animal husbandry particularly ruminants to help reducing CO2 from environment > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 11,439,604 B1 - Document ID: US11439604B1 - Status: Granted - Kind: B1 - Application number: 17/466,644 - Filed: 2021-09-03 - Granted: 2022-09-13 - Earliest claimed: 2021-03-03 - Applicants: Manuel Serrano - Inventors: Manuel Serrano - Web page: https://patentgrep.com/patent/US11439604B1 ## CPC - A61K 31/05: Phenols {(cannabinoids A61K31/658)} - A23K 10/14: Pretreatment of feeding-stuffs with enzymes - A23K 10/30: from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms (obtained by microbiological or biochemical processes, e.g. using yeasts or enzymes, A23K10/10) - A23K 20/105: Aliphatic or alicyclic compounds - A23K 20/111: Aromatic compounds - A23K 20/158: Fatty acids; Fats; Products containing oils or fats - A23K 20/174: Vitamins - A23K 20/179: Colouring agents, e.g. pigmenting or dyeing agents - A23K 40/10: by agglomeration; by granulation, e.g. making powders - A23K 50/10: for ruminants - A61K 9/0056: {Mouth soluble or dispersible forms; Suckable, eatable, chewable coherent forms; Forms rapidly disintegrating in the mouth; Lozenges; Lollipops; Bite capsules; Baked products; Baits or other oral forms for animals} - A61K 47/44: Oils, fats or waxes according to two or more groups of A61K47/02-A61K47/42; Natural or modified natural oils, fats or waxes, e.g. castor oil, polyethoxylated castor oil, montan wax, lignite, shellac, rosin, beeswax or lanolin (synthetic glycerides, e.g. medium-chain triglycerides, A61K47/14) - C12P 7/22: aromatic - Y02P 60/22: Methane [CH4], e.g. from rice paddies - Y02P 60/87: Re-use of by-products of food processing for fodder production - A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D) - A23K: FODDER - C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)} - Y02P: CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS ## Abstract Feed additive including sodium carbonate feed additive polyphenol particles can be fed to animals to reduce methane or carbon dioxide emission. The feed additive polyphenol particles can be prepared by reacting phenols with sodium percarbonate (2Na2CO3.3H2O2) in a solution of water, a peroxidase and a dispersing agent. The sodium percarbonate can be dissolved in the water solution to generate sodium and carbonate ions, which can be incorporated to the polyphenol particles for assisting the animals in digesting the feed additive. The dispersing agent can be used to limit the sizes of the particles to facilitate the consumption of the feed additive by the animals. ## Claims ### Claim 1 A method of forming particles for an animal feed additive for reducing carbon dioxide emission from the animal, the method comprising forming a first solution comprising a phenol dissolved in a solvent; wherein the phenol comprises a polyphenol extracted from pomegranate, apple peel, garlic or yucca, wherein the solvent comprises an aromatic hydrocarbon, mixing the first solution with a second solution comprising an oxidant, an enzyme, and a dispersing agent, wherein the enzyme comprises horse radish peroxidase or amylase, wherein the oxidant comprises sodium percarbonate, wherein the oxidant is configured to oxidize the phenol with the enzyme acting as a catalyst, wherein the dispersing agent is configured to limit sizes of particles coalescing from the oxidation of the phenol; wherein the dispersing agent comprises a grain or seed oil, and removing the particles from the mixture. ### Claim 2 (depends on 1) A method as in claim 1, wherein the mixture is formed by gradually adding the second solution, wherein the second solution is added at a rate which is approximately equal to the rate of consumption of the oxidant. ### Claim 3 (depends on 1) A method as in claim 1, wherein a ratio of the phenol to the dispersing agent is between 3 to 1 and 1 to 3, wherein a ratio of the phenol to the oxidant is between 0.1 to 2.5 moles of oxidant per 100 grams of phenol, and wherein a ratio of the phenol to the enzyme is between 10 mg to 5 g of enzyme per 100 grams of phenol. ### Claim 4 (depends on 1) A method as in claim 1, further comprising adding seaweed, bromo chloromethane, 2-Bromoethanol sulfate, ciclodextrine and asparagopsis taxiformis or asparagopsis armata to the mixture. ### Claim 5 (depends on 1) A method as in claim 1, wherein the dispersing agent amount is configured to form particles having a predetermined size between 5 and 7 cm. ### Claim 6 (depends on 1) A method as in claim 1, wherein the feed additive further comprises feed grains, flavorings, colorants, micronutrients, or vitamins, wherein the feed additive further comprises seaweed, bromo chloromethane, 2-Bromoethanol sulfate, ciclodextrine and asparagopsis taxiformis or asparagopsis armata, wherein the feed additive is configured to reduce carbon dioxide upon oral consumption of the feed additive by the animal.