# US 11,439,327 B2 · Apparatus and method for measuring Raman spectrum

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- Number: US 11,439,327 B2
- Document ID: US11439327B2
- Status: Granted
- Kind: B2
- Application number: 16/816,489
- Filed: 2020-03-12
- Published: 2020-12-24
- Granted: 2022-09-13
- Earliest claimed: 2019-06-18
- Assignees: Samsung Electronics Co., Ltd.
- Applicants: Samsung Electronics Co., Ltd.
- Inventors: Yun S Park; Woo Chang Lee; Ho Jun Chang
- Web page: https://patentgrep.com/patent/US11439327B2

## CPC

- A61B 5/14532: {for measuring glucose, e.g. by tissue impedance measurement}
- A61B 5/1455: using optical sensors, e.g. spectral photometrical oximeters
- G01J 3/0237: {Adjustable, e.g. focussing}
- G01J 3/027: {Control of working procedures of a spectrometer; Failure detection; Bandwidth calculation}
- G01J 3/0278: {Control or determination of height or angle information for sensors or receivers}
- G01J 3/44: Raman spectrometry; Scattering spectrometry {; Fluorescence spectrometry}
- G01J 3/4412: {Scattering spectrometry (particle sizing by light scattering G01N15/0205; optical velocimetry of particles G01P5/20, G01P5/26)}
- G01N 21/65: Raman scattering
- G01N 2021/656: {Raman microprobe}
- A61B: DIAGNOSIS; SURGERY; IDENTIFICATION (usefulness limited to only animals A61D)
- G01J: MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY (light sources F21, H01J, H01K, H05B; investigating properties of materials by optical means G01N)
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

An apparatus for measuring a Raman spectrum may include a processor configured to adjust a Raman probe parameter, set a Raman probe with the Raman probe parameter, obtain a first Raman spectrum of the sample at a first time point and a second Raman spectrum of the sample at a second time point, obtain a difference spectrum between the first Raman spectrum and the second Raman spectrum, determine a degree of similarity between the difference spectrum and an analyte Raman spectrum, determine an optimal Raman probe parameter based on the degree of similarity, and obtain a Raman spectrum of the sample for measuring bio-information by setting the Raman probe with the optimal Raman probe parameter.

## Claims

### Claim 1

An apparatus for measuring a Raman spectrum, the apparatus comprising:
a processor configured to:
adjust a Raman probe parameter;
set a Raman probe with the Raman probe parameter;
obtain a first Raman spectrum of the sample at a first time point and a second Raman spectrum of the sample at a second time point;
obtain a difference spectrum between the first Raman spectrum and the second Raman spectrum;
determine a degree of similarity between the difference spectrum and an analyte Raman spectrum;
determine an optimal Raman probe parameter based on the degree of similarity; and
obtain a Raman spectrum of the sample for measuring bio-information by setting the Raman probe with the optimal Raman probe parameter.

### Claim 2 (depends on 1)

The apparatus of claim 1, wherein the processor is configured to determine that the Raman probe parameter that maximizes the degree of similarity is the optimal Raman probe parameter by iteratively repeating operations of adjusting the Raman probe parameter, obtaining the first Raman spectrum and the second Raman spectrum, obtaining the difference spectrum, and determining the degree of similarity.

### Claim 3 (depends on 1)

The apparatus of claim 1, wherein the processor is configured to determine that the Raman probe parameter is the optimal Raman probe parameter based on determining that the degree of similarity exceeds a predetermined threshold value.

### Claim 4 (depends on 3)

The apparatus of claim 3, wherein in response to determining that the degree of similarity is less than or equal to the predetermined threshold value, the processor is configured to repeat operations of adjusting the Raman probe parameter, obtaining the first Raman spectrum and the second Raman spectrum, obtaining the difference spectrum, and determining the degree of similarity until the degree of similarity between the difference spectrum and the analyte Raman spectrum exceeds the predetermined threshold value.

### Claim 5 (depends on 1)

The apparatus of claim 1, wherein the Raman probe parameter includes at least one of a spacing between a light collector of the Raman probe and a sample, and a focal depth of the light collector.

### Claim 6 (depends on 1)

The apparatus of claim 1, wherein the analyte Raman spectrum is a Raman spectrum of glucose solution.

### Claim 7 (depends on 1)

The apparatus of claim 1, wherein the processor is configured to determine the degree of similarity using at least one of Euclidean distance, Manhattan Distance, cosine distance, Mahalanobis Distance, Jaccard coefficient, extended Jaccard coefficient, Pearson's correlation coefficient, and Spearman's correlation coefficient.

### Claim 8 (depends on 1)

The apparatus of claim 1, wherein the bio-information is a concentration of analyte, and the analyte includes at least one of glucose, triglycerides, urea, uric acids, lactate, proteins, cholesterol, antioxidants, and ethanol.

### Claim 9 (depends on 1)

The apparatus of claim 1, wherein the apparatus further comprises the Raman probe comprising:
a light source configured to emit light to a sample;
a light collector configured to collect Raman scattered light from the sample;
a spectrometer configured to divide the Raman scattered light collected in the light collector based on wavelength; and
a photodetector configured to receive the Raman scattered light divided based on wavelength.

### Claim 10

A method of measuring a Raman spectrum, the method comprising:
adjusting a Raman probe parameter;
setting a Raman probe with the Raman probe parameter;
obtaining a first Raman spectrum of a sample at a first time point and a second Raman spectrum of the sample at a second time point;
obtaining a difference spectrum between the first Raman spectrum and the second Raman spectrum;
determining a degree of similarity between the difference spectrum and an analyte Raman spectrum;
determining an optimal Raman probe parameter based on the degree of similarity; and
obtaining a Raman spectrum of the sample for measuring bio-information by setting the Raman probe with the optimal Raman probe parameter.

### Claim 11 (depends on 10)

The method of claim 10, wherein the determining of the optimal Raman probe parameter comprises determining that the Raman probe parameter that maximizes the degree of similarity is the optimal Raman probe parameter by iteratively repeating the operations of adjusting the Raman probe parameter, obtaining the first Raman spectrum and the second Raman spectrum, obtaining the difference spectrum, and determining the degree of similarity.

### Claim 12 (depends on 10)

The method of claim 10, wherein the determining of the optimal Raman probe parameter comprises determining that the Raman probe parameter is the optimal Raman probe parameter based on determining that the degree of similarity exceeds a predetermined threshold value.

### Claim 13 (depends on 12)

The method of claim 12, wherein in response to determining that the degree of similarity is less than or equal to the threshold value, the determining of the optimal Raman probe parameter comprises iteratively repeating the operations of adjusting the Raman probe parameter, obtaining the first Raman spectrum and the second Raman spectrum, obtaining the difference spectrum, and determining the degree of similarity until the degree of similarity between the difference spectrum and the analyte Raman spectrum exceeds the predetermined threshold value.

### Claim 14 (depends on 10)

The method of claim 10, wherein the Raman probe parameter includes at least one of a spacing between a light collector of the Raman probe and the sample, and a focal depth of the light collector.

### Claim 15 (depends on 10)

The method of claim 10, wherein the analyte Raman spectrum is a Raman spectrum of glucose solution.

### Claim 16 (depends on 10)

The method of claim 10, wherein the determining of the degree of similarity comprises determining the degree of similarity using at least one of Euclidean distance, Manhattan Distance, cosine distance, Mahalanobis Distance, Jaccard coefficient, extended Jaccard coefficient, Pearson's correlation coefficient, and Spearman's correlation coefficient.

### Claim 17 (depends on 10)

The method of claim 10, wherein the bio-information is a concentration of an analyte, and the analyte includes at least one of glucose, triglycerides, urea, uric acids, lactate, proteins, cholesterol, antioxidants, and ethanol.