# US 11,439,326 B2 · Analyte monitoring: stabilizer for subcutaneous glucose sensor with incorporated antiglycolytic agent > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 11,439,326 B2 - Document ID: US11439326B2 - Status: Granted - Kind: B2 - Application number: 16/252,994 - Filed: 2019-01-21 - Published: 2019-05-23 - Granted: 2022-09-13 - Earliest claimed: 2005-12-28 - Assignees: Abbott Diabetes Care Inc. - Applicants: Abbott Diabetes Care Inc. - Inventors: Tianmei Ouyang; Zenghe Liu; Hyun Cho; Benjamin J. Feldman - Web page: https://patentgrep.com/patent/US11439326B2 ## CPC - A61B 5/14532: {for measuring glucose, e.g. by tissue impedance measurement} - A61B 5/1473: invasive, e.g. introduced into the body by a catheter - A61B 5/1477: non-invasive - A61B 5/14865: {invasive, e.g. introduced into the body by a catheter or needle or using implanted sensors} - A61B 5/0031: {Implanted circuitry} - A61B: DIAGNOSIS; SURGERY; IDENTIFICATION (usefulness limited to only animals A61D) ## Abstract An analyte sensor including an antiglycolytic agent or a precursor thereof and a chelating agent that stabilizes the antiglycolytic agent positioned proximate to the working electrode of the sensor. Also provided are systems and methods of using the electrochemical analyte sensors in analyte monitoring. ## Claims ### Claim 1 A glucose sensor comprising: a) a working electrode comprising a sensing layer disposed thereon, the sensing layer comprising a glucose responsive enzyme; b) a counter electrode; and c) a coating comprising an active agent and a chelating agent, the coating positioned proximate to the working electrode, wherein the active agent comprises an antiglycolytic agent, and wherein the chelating agent stabilizes the active agent. ### Claim 2 (depends on 1) The glucose sensor of claim 1, wherein the active agent is chemically bonded to the chelating agent. ### Claim 3 (depends on 1) The glucose sensor of claim 1, wherein the active agent is selected from the group consisting of a fluoride, a glyceraldehyde, mannose, glucosamine, mannoheptulose, sorbose-6-phophate, trehalose-6-phosphate, maleimide, an iodoacetate, and any combination thereof. ### Claim 4 (depends on 1) The glucose sensor of claim 1, wherein the active agent is a glyceraldehyde. ### Claim 5 (depends on 4) The glucose sensor of claim 4, wherein the glyceraldehyde is present in a range of from about 1 microgram to about 2 milligrams. ### Claim 6 (depends on 1) The glucose sensor of claim 1, wherein the chelating agent is selected from the group consisting of borate, boric acid, a main group metal, a transition group metal, a group IIIA metal, and any combination thereof. ### Claim 7 (depends on 1) The glucose sensor of claim 1, wherein the chelating agent is boric acid. ### Claim 8 (depends on 7) The glucose sensor of claim 7, wherein the boric acid is present in a range of from about 0.1 microgram to about 1 milligram. ### Claim 9 (depends on 1) The glucose sensor of claim 1, wherein the sensing layer comprises an electron transfer agent. ### Claim 10 A glucose sensor comprising: a) a working electrode comprising a sensing layer disposed thereon, the sensing layer comprising a glucose responsive enzyme; b) a counter electrode; and c) a membrane disposed over at least a portion of the sensing layer, the membrane comprising an active agent and a chelating agent, wherein the active agent comprises an antiglycolytic agent, and wherein the chelating agent stabilizes the active agent. ### Claim 11 (depends on 10) The glucose sensor of claim 10, wherein the active agent is chemically bonded to the chelating agent. ### Claim 12 (depends on 10) The glucose sensor of claim 10, wherein the active agent is selected from the group consisting of a fluoride, a glyceraldehyde, mannose, glucosamine, mannoheptulose, sorbose-6-phophate, trehalose-6-phosphate, maleimide, an iodoacetate, and any combination thereof. ### Claim 13 (depends on 10) The glucose sensor of claim 10, wherein the active agent is a glyceraldehyde. ### Claim 14 (depends on 13) The glucose sensor of claim 13, wherein the glyceraldehyde is present in a range of from about 1 microgram to about 2 milligrams. ### Claim 15 (depends on 10) The glucose sensor of claim 10, wherein the chelating agent is selected from the group consisting of borate, boric acid, a main group metal, a transition group metal, a group IIIA metal, and any combination thereof. ### Claim 16 (depends on 10) The glucose sensor of claim 10, wherein the chelating agent is boric acid. ### Claim 17 (depends on 16) The glucose sensor of claim 16, wherein the boric acid is present in a range of from about 0.1 microgram to about 1 milligram. ### Claim 18 (depends on 10) The glucose sensor of claim 10, wherein the sensing layer comprises an electron transfer agent. ### Claim 19 (depends on 10) The glucose sensor of claim 10, wherein at least the active agent is time released from the membrane. ### Claim 20 A glucose sensor comprising: a) a working electrode comprising a sensing layer disposed thereon, the sensing layer comprising a glucose responsive enzyme; b) a counter electrode; and c) an active agent and a chelating agent positioned proximate to the working electrode, wherein the active agent comprises an antiglycolytic agent, and wherein the active agent is chemically bonded to the chelating agent. ### Claim 21 A glucose sensor comprising: a) a working electrode comprising a sensing layer disposed thereon, the sensing layer comprising a glucose responsive enzyme; b) a counter electrode; and c) a coating comprising an active agent and a chelating agent, the coating positioned proximate to the working electrode, wherein the active agent comprises an antiglycolytic agent or precursor thereof, and wherein the chelating agent stabilizes the active agent. ### Claim 22 A glucose sensor comprising: a) a working electrode comprising a sensing layer disposed thereon, the sensing layer comprising a glucose responsive enzyme; b) a counter electrode; and c) a membrane disposed over at least a portion of the sensing layer, the membrane comprising an active agent and a chelating agent, wherein the active agent comprises an antiglycolytic agent or precursor thereof, and wherein the chelating agent stabilizes the active agent. ### Claim 23 A glucose sensor comprising: a) a working electrode comprising a sensing layer disposed thereon, the sensing layer comprising a glucose responsive enzyme; b) a counter electrode; and c) an active agent and a chelating agent positioned proximate to the working electrode, wherein the active agent comprises an antiglycolytic agent or precursor thereof, and wherein the active agent is chemically bonded to the chelating agent.