# US 11,439,073 B2 · Interspecific hybrid plant resulting from a cross between an Allium ampeloprasum plant with an Allium sativum plant

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- Number: US 11,439,073 B2
- Document ID: US11439073B2
- Status: Granted
- Kind: B2
- Application number: 16/476,691
- Filed: 2018-01-17
- Published: 2020-08-20
- Granted: 2022-09-13
- Earliest claimed: 2017-01-19
- Assignees: Bejo Zaden B.V.
- Applicants: Bejo Zaden B.V.
- Inventors: Kai Ament; Henricus Chretien Marie Louise Bongers; Marcel Adriaanse; Albertus Johannes Maria Schrijver
- Web page: https://patentgrep.com/patent/US11439073B2

## CPC

- A01H 1/021: {Methods of breeding using interspecific crosses, i.e. interspecies crosses}
- A01H 1/045: {using molecular markers}
- A01H 5/04: Stems
- A01H 5/06: Roots
- A01H 5/12: Leaves
- A01H 6/04: Amaryllidaceae, e.g. onion
- C12Q 1/6895: for plants, fungi or algae
- C12Q 2600/13: Plant traits
- A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES

## Abstract

The present invention relates to a hybrid between a leek and a garlic plant. More specifically, the invention relates to an interspecific hybrid plants resulting from a cross between an Allium ampeloprasum plant and an Allium sativum plant. Specifically, the present invention relates to Interspecific hybrid plants derived from a cross between an Allium ampeloprasum plant and an Allium sativum plant, wherein the hybrid plants are capable of producing seed and contains nuclear genomic material derived from both said Allium ampeloprasum plant and said Allium sativum plant, and further wherein the interspecific hybrid plants contain at least 250 mg/kg of allicin when determined in the white part of the leaf sheet of the plants and furthermore wherein the interspecific hybrid plants are obtainable, obtained or derived, from an interspecific hybrid plant as deposited under accession number NCIMB 42564.

## Claims

### Claim 1

An interspecific hybrid plant derived from a cross between an Allium ampeloprasum plant and an Allium sativum plant, wherein said interspecific hybrid plant is capable of producing seed, wherein said interspecific hybrid plant is heterozygous in its genome for at least 50 markers selected from the sequences of SEQ ID NO: 1 to SEQ ID NO: 65, each of the at least 50 markers comprising a garlic-leek interspecific single nucleotide polymorphism (SNP), wherein said interspecific hybrid plant contains at least 250 mg/kg of allicin when determined in a white part of a leaf sheet of the plant, and wherein said interspecific hybrid plant is grown from a seed of an interspecific hybrid plant as deposited under accession number NCIMB 42564.

### Claim 2 (depends on 1)

The interspecific hybrid plant according to claim 1, wherein the interspecific hybrid plant is heterozygous in its genome for each of the markers represented by SEQ ID NO: 1 to SEQ ID NO: 65, each of the markers comprising a garlic-leek interspecific single nucleotide polymorphism (SNP).

### Claim 3 (depends on 1)

The interspecific hybrid plant according to claim 1, wherein at least one odor compound selected from the group consisting of methyl allyl disulfide, propenyl methyl disulfide and diallyl disulfide is present in the white part of the leaf sheet of the plant.

### Claim 4 (depends on 3)

The interspecific hybrid plant according to claim 3, wherein
the amount of methyl allyl disulfide is at least 25 mg/kg as determined in the white part of the leaf sheet of the plant, and/or
the amount of diallyl disulfide is at least 50 mg/kg as determined in the white part of the leaf sheet of the plant.

### Claim 5 (depends on 1)

The interspecific hybrid plant according to claim 1, wherein the plant is male sterile.

### Claim 6 (depends on 1)

Plant parts, edible parts, protoplasts, callus, cultured cells, cultured tissues of the interspecific hybrid plant according to claim 1.

### Claim 7

A seed of an interspecific hybrid plant derived from a cross between an Allium ampeloprasum plant and an Allium sativum plant, wherein the seed is heterozygous in its genome for at least 50 markers selected from the sequences of SEQ ID NO: 1 to SEQ ID NO: 65, each of the at least 50 markers comprising a garlic-leek interspecific single nucleotide polymorphism (SNP), and wherein the seed has been deposited with NCIMB under Accession No. 42564.