Method for detecting mycoplasma using mitochondrial DNA as internal control sample

A method for providing information for diagnosing mycoplasma infection, comprising:

• i) extracting DNA from a host cell isolated from a subject suspected of being infected with mycoplasma;
• ii) performing polymerase chain reaction (PCR) using the DNA extracted in step i) as a template;
• and
• iii) determining that the subject is infected with mycoplasma, if the amplification of mycoplasma DNA is confirmed in the PCR of step ii),
• wherein the PCR of step ii) amplifies a target sequence using a primer set for amplifying mycoplasma DNA;
• and a primer set for amplifying mitochondrial DNA of a host cell, wherein the primer set for amplifying mitochondrial DNA of a host cell comprises:
• a forward primer comprising a nucleotide sequence of SEQ ID NO:
• 10;
• and a reverse primer comprising a nucleotide sequence of SEQ ID NO:
• 11.

A kit for detecting mycoplasma, comprising:

• a) a cell lysis buffer;
• b) a primer set for amplifying mycoplasma DNA;
• and
• c) a primer set for amplifying mitochondrial DNA of a host cell, wherein the primer set for amplifying mitochondrial DNA of a host cell comprises:
• a forward primer comprising a nucleotide sequence of SEQ ID NO:
• 10;
• and
• a reverse primer comprising a nucleotide sequence of SEQ ID NO:
• 11.