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US 11,434,506 B2Granted
Production of desaturated fatty alcohols and desaturated fatty alcohol acetates in yeast
AssigneeDanmarks Tekniske Universitet
InventorsIrina Borodina, Carina Holkenbrink, Marie Inger Dam, Christer Löfstedt, Baojian Ding, Hong-Lei Wang
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Abstract
The present invention relates to the production of compounds comprised in pheromones, in particular moth pheromones, such as desaturated fatty alcohols and desaturated fatty alcohol acetates and derivatives thereof, from a yeast cell.
Claims18
A yeast cell capable of producing desaturated fatty alcohols, said yeast cell expressing:
- i) at least one heterologous fatty acyl-CoA desaturase capable of introducing at least one double bond in a fatty acyl-CoA having a carbon chain length of 14, wherein said desaturase is selected from the group consisting of a Δ9 desaturase and a Δ11 desaturase;
- and:
- a. has an amino acid sequence having at least 80% homology to the Δ9 desaturase from Drosophila melanogaster as set forth in SEQ ID NO:
- 10;
- b. has an amino acid sequence having at least 80% homology to the Δ9 desaturase from Spodoptera litura as set forth in SEQ ID NO:
- 12;
- c. has an amino acid sequence having at least 80% homology to the Δ11 desaturase from Choristoneura parallela as set forth in SEQ ID NO:
- 66;
- or
- d. has an amino acid sequence having at least 80% homology to the Δ11 desaturase from Choristoneura rosaceana as set forth in SEQ ID NO:
- 65;
- and
- ii) at least one heterologous fatty acyl-CoA reductase (FAR), capable of converting at least part of said desaturated fatty acyl-CoA to a desaturated fatty alcohol
- wherein the fatty acyl-CoA desaturase has a higher specificity towards tetradecanoyl-CoA than towards hexadecanoyl-CoA and/or wherein the fatty acyl-CoA reductase has a higher specificity towards desaturated tetradecanoyl-CoA than towards desaturated hexadecanoyl-CoA and wherein at least 10% of the desaturated fatty alcohols have a carbon chain length of 14.
11 more elements
Dependencies are inferred from the claim text.