Recombinant vector comprising BiP fragment and preparation method of recombinant proteins using thereof
AssigneeBioapplications Inc.
InventorYongjik Lee
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Abstract
Provided is a recombinant vector including a new BiP gene fragment, and when a target protein is prepared using the recombinant vector of the subject matter, use stability can be enhanced and the production amount of target protein can also be increased by minimizing a foreign peptide sequence remaining in the target protein.
Claims22
A gene construct in which a fragment of chaperone binding protein (BiP) gene consisting of the nucleotide sequence of SEQ ID NO. 6 and a polynucleotide encoding a target protein are sequentially linked so as to be operable, wherein the gene construct does not contain a nucleic acid sequence corresponding to positions 252-272 of SEQ ID NO:2 at a 3′ terminal side immediately after the BiP gene fragment represented by SEQ ID NO. 6.
A recombinant vector comprising a gene construct in which a promoter gene, a fragment of BiP gene consisting of the nucleotide sequence of SEQ ID NO. 6, and a polynucleotide encoding a target protein are sequentially linked so as to be operable, wherein the gene construct does not contain a nucleic acid sequence corresponding to positions 252-272 of SEQ ID NO:2 at a 3′ terminal side immediately after BiP gene fragment represented by SEQ ID NO. 6.
Dependencies are inferred from the claim text.