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US 11,434,492 B2Granted
Ribosome-mediated incorporation of peptides and peptidomimetics
AssigneeArizona Board of Regents on behalf of Arizona State University
InventorsSidney Hecht, Larisa (Liza) Dedkova, Rumit Maini, Sandipan Roy Chowdhury, Rakesh Paul
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Abstract
Modified ribosomes that were selected using a dipeptidyl-puromycin aminonucleoside are used to mediate site-specific incorporation of one or more peptides and peptidomimetics into protein in a cell free translation system. In addition, new fluorescent dipeptidomimetics have been synthesized and incorporated into proteins, as well as modified proteins containing one or more non-naturally occurring dipeptides.
Claims4
An in vitro translation system comprising:
- a transfer RNA (tRNA) comprising a modified dipeptidomimetic selected from
- and
- a genetically modified Escherichia coli (E. coli) ribosome, the modification of the genetically modified ribosome consisting of:
- a 23S ribosomal RNA (rRNA) sequence consisting of a modified Region 1 (position 2057-2063) selected from UGCGUGG or AGCGUGA, and a modified Region 2 (position 2502-2507), wherein:
- (a) if the modified Region 1 consists of UGCGUGG from position 2057-2063, the modified Region 2 is selected from the group consisting of ACGAAG, CGCACG, CUAUGU, CGCAAU, or CUACAG from position 2502-2507;
- (b) if the modified Region 1 consists of AGCGUGA from position 2057-2063, the modified Region 2 is selected from the group consisting of CUGCGU, UGGCAG, AUCAGG, or AUCCGA from position 2502-2507.
3 more elements
Dependencies are inferred from the claim text.